Guzen Fausto Pierdoná, de Araújo Dayane Pessoa, Lucena Eudes Euler de Souza, de Morais Hécio Henrique Araújo, Cavalcanti José Rodolfo Lopes de Paiva, do Nascimento Expedito Silva, Costa Miriam Stela Maris de Oliveira, Cavalcante Jeferson Sousa
Laboratory of Experimental Neurology, Health Science Center, State University of Rio Grande do Norte, Mossoró, RN, Brazil.
Laboratory of Experimental Neurology, Health Science Center, State University of Rio Grande do Norte, Mossoró, RN, Brazil.
Neurosci Lett. 2016 Mar 11;616:43-8. doi: 10.1016/j.neulet.2015.08.043. Epub 2015 Aug 28.
Neurotrophic factors and peripheral nerves are known to be good substrates for bridging CNS trauma. The involvement of fibroblast growth factor-2 (FGF-2) activation in the dorsal root ganglion (DRG) was examined following spinal cord injury in the rat. We evaluated whether FGF-2 increases the ability of a sciatic nerve graft to enhance neuronal plasticity, in a gap promoted by complete transection of the spinal cord. The rats were subjected to a 4mm-long gap at low thoracic level and were repaired with saline (Saline or control group, n=10), or fragment of the sciatic nerve (Nerve group, n=10), or fragment of the sciatic nerve to which FGF-2 (Nerve+FGF-2 group, n=10) had been added immediately after lesion. The effects of the FGF-2 and fragment of the sciatic nerve grafts on neuronal plasticity were investigated using choline acetyl transferase (ChAT)-immunoreactivity of neurons in the dorsal root ganglion after 8 weeks. Preservation of the area and diameter of neuronal cell bodies in dorsal root ganglion (DRG) was seen in animals treated with the sciatic nerve, an effect enhanced by the addition of FGF-2. Thus, the addition of exogenous FGF-2 to a sciatic nerve fragment grafted in a gap of the rat spinal cord submitted to complete transection was able to improve neuroprotection in the DRG. The results emphasized that the manipulation of the microenvironment in the wound might amplify the regenerative capacity of peripheral neurons.
神经营养因子和周围神经被认为是桥接中枢神经系统创伤的良好底物。在大鼠脊髓损伤后，研究了背根神经节（DRG）中纤维母细胞生长因子-2（FGF-2）激活的情况。我们评估了在脊髓完全横断造成的间隙中，FGF-2是否能增强坐骨神经移植物促进神经元可塑性的能力。将大鼠在胸段低位造成4毫米长的间隙，分别用生理盐水修复（生理盐水组或对照组，n = 10）、坐骨神经片段修复（神经组，n = 10）或损伤后立即添加FGF-2的坐骨神经片段修复（神经+FGF-2组，n = 10）。8周后，利用背根神经节中神经元的胆碱乙酰转移酶（ChAT）免疫反应性，研究FGF-2和坐骨神经移植物片段对神经元可塑性的影响。在用坐骨神经治疗的动物中，观察到背根神经节（DRG）中神经元细胞体的面积和直径得以保留，添加FGF-2可增强这种效果。因此，在完全横断的大鼠脊髓间隙中移植的坐骨神经片段添加外源性FGF-2，能够改善DRG中的神经保护作用。结果强调，对伤口微环境的操控可能会增强周围神经元的再生能力。
