The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China.
The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China.
Biomed Pharmacother. 2015 Oct;75:137-41. doi: 10.1016/j.biopha.2015.07.020. Epub 2015 Aug 28.
The expression and roles of MicroRNA-381 (miR-381) has been explored in several types of human cancers. However, its biological functions in colon cancer remain largely unknown. Quantitative real-time PCR assays were used to detect the expression of miR-381 in human colon cancer tissues and adjacent normal tissues. miR-381 antisense oligos and mimics were introduced into SW480 and HCT116 cells. Bioinformatic prediction analysis was performed to identify the potential targets of miR-381. Protein expression analysis, luciferase assays and rescue assays were used to confirm the substrate of miR-381. We found that miR-381 was significantly down-regulated in human colon cancer tissues, compared with adjacent normal tissues. Introduction of miR-381 antisense oligos into SW480 and HCT116 cells promoted cell proliferation and invasion. Besides, inhibition of miR-381 could also support tumor growth in the nude mice. Additionally, bioinformatic prediction suggested that the nuclear receptor liver receptor homologue 1 (LRH-1) is a target gene of miR-381. Thus, our data suggested that down-regulation of miR-381 plays an important role in the colon cancer progression.
MicroRNA-381 (miR-381) 的表达和作用已在几种人类癌症中得到探索。然而，其在结肠癌中的生物学功能在很大程度上仍不清楚。定量实时 PCR 检测用于检测人结肠癌组织和相邻正常组织中 miR-381 的表达。将 miR-381 反义寡核苷酸和模拟物引入 SW480 和 HCT116 细胞。通过生物信息学预测分析鉴定 miR-381 的潜在靶标。蛋白表达分析、荧光素酶报告基因实验和挽救实验用于确认 miR-381 的底物。我们发现 miR-381 在人结肠癌组织中明显下调，与相邻正常组织相比。将 miR-381 反义寡核苷酸导入 SW480 和 HCT116 细胞促进细胞增殖和侵袭。此外，抑制 miR-381 还可以支持裸鼠中的肿瘤生长。此外，生物信息学预测表明核受体肝受体同源物 1 (LRH-1) 是 miR-381 的靶基因。因此，我们的数据表明 miR-381 的下调在结肠癌进展中起着重要作用。
