通过定量p53免疫组织化学评估年轻乳腺癌女性的生存预后
Prognosis for Survival of Young Women with Breast Cancer by Quantitative p53 Immunohistochemistry.
作者信息
Axelrod David E, Shah Kinsuk, Yang Qifeng, Haffty Bruce G
机构信息
Department of Genetics and Cancer Institute of New Jersey, Rutgers University, New Jersey, USA.
Department of Genetics, Rutgers University, USA.
出版信息
Cancer Clin Oncol. 2012;1(1):52-64. doi: 10.5539/cco.v1n1p52.
p53 protein detected immunohistochemically has not been accepted as a biomarker for breast cancer patients because of disparate reports of the relationship between the amount of p53 protein detected and patient survival. The purpose of this study was to determine experimental conditions and methods of data analysis for which p53 stain intensity could be prognostic for survival of young breast cancer patients. A tissue microarray of specimens from 93 patients was stained with anti-p53 antibody, and stain intensity measured with a computer-aided image analysis system. A cut-point at one standard deviation below the mean of the distribution of p53 stain intensity separated patients into two groups with significantly different survival. These results were confirmed by Quantitative Nuclear Grade determined by DNA-specific Feulgen staining. P53 provided information beyond ER and PR status. Therefore, under the conditions reported here, p53 protein can be an effective prognostic factor for young breast cancer patients.
免疫组织化学检测到的p53蛋白尚未被公认为乳腺癌患者的生物标志物，因为关于检测到的p53蛋白量与患者生存率之间关系的报道存在差异。本研究的目的是确定实验条件和数据分析方法，在此条件下p53染色强度可作为年轻乳腺癌患者生存的预后指标。用抗p53抗体对93例患者的组织芯片标本进行染色，并用计算机辅助图像分析系统测量染色强度。p53染色强度分布均值以下一个标准差处的切点将患者分为两组，其生存率有显著差异。这些结果通过DNA特异性福尔根染色确定的定量核分级得到证实。p53提供了超出雌激素受体（ER）和孕激素受体（PR）状态的信息。因此，在此处报道的条件下，p53蛋白可以成为年轻乳腺癌患者有效的预后因素。
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