Liu Xing, Gong Xiao-jian, Chen Hua-guo, Zhou Mei, Zhou Xin
Zhongguo Zhong Yao Za Zhi. 2015 May;40(9):1771-5.
The method was established for determination of geniposidic acid, chlorogenic acid, pinoresinoldiglucoside, which are three kinds of constituents of Eucommia ulmoides absorbed into the blood components. LC-MS/MS technique was applied to determine the blood components of the bloodstream after administration of E. ulmoides extract. At the same time, HPLC was used for detection of the ingredients content of the blood sample from 23 batches of E. ulmoides. The results showed that geniposidic acid, chlorogenic acid and pinoresinoldiglucoside are prototype into the blood in rats after oral administration of E. ulmoides extract, The linear range of geniposidic acid, chlorogenic acid and pinoresinoldiglucoside was good, and the average recoveries geniposidic acid, chlorogenic acid and pinoresinoldiglucoside were 98.69%, 100.8% and 98.39%, respectively. The method is simple and feasible with good reproducibility.
建立了测定杜仲吸收进入血液成分中的三种成分（京尼平苷酸、绿原酸、松脂醇二葡萄糖苷）的方法。采用液相色谱 - 串联质谱技术测定杜仲提取物给药后血液中的成分。同时，用高效液相色谱法检测23批杜仲血样中的成分含量。结果表明，大鼠口服杜仲提取物后，京尼平苷酸、绿原酸和松脂醇二葡萄糖苷均以原型入血，京尼平苷酸、绿原酸和松脂醇二葡萄糖苷的线性范围良好，其平均回收率分别为98.69%、100.8%和98.39%。该方法简便可行，重现性好。
