Nalini S, Balasubramanian K A
Indian J Biochem Biophys. 1989 Dec;26(6):357-60.
Individual nonesterified fatty acids were bound to albumin in vitro and these fatty acid albumin complexes were used to study their effect on lipid peroxidation in liver microsomes. Peroxidation was induced by various methods and malondialdehyde (MDA) was measured as an index of peroxidation. Among the fatty acids tested, albumin-bound monounsaturated fatty acids showed more inhibition of peroxidation as compared to other fatty acids. Increasing the concentration of iron in the peroxidizing system, partially reversed the inhibition by fatty acids. Moreover, albumin-bound fatty acid did not inhibit iron independent peroxidation. This suggests that, like nonesterified fatty acids, albumin-bound fatty acids inhibit peroxidation by chelating the iron. Albumin fatty acid complex, similar to the fatty acid composition present in the circulating albumin, also showed inhibition of peroxidation. These data indicate that nonesterified fatty acids even when bound to albumin are capable of inhibiting peroxidation and circulating albumin, which contains various fatty acids bound to it, may impart some antioxidant effect in addition to other plasma antioxidants.
在体外，将单个非酯化脂肪酸与白蛋白结合，然后利用这些脂肪酸 - 白蛋白复合物研究它们对肝微粒体脂质过氧化的影响。通过各种方法诱导过氧化反应，并测定丙二醛（MDA）作为过氧化反应的指标。在所测试的脂肪酸中，与其他脂肪酸相比，白蛋白结合的单不饱和脂肪酸对过氧化反应的抑制作用更强。增加过氧化体系中铁的浓度，部分逆转了脂肪酸的抑制作用。此外，白蛋白结合的脂肪酸并不抑制与铁无关的过氧化反应。这表明，与非酯化脂肪酸一样，白蛋白结合的脂肪酸通过螯合铁来抑制过氧化反应。与循环白蛋白中存在的脂肪酸组成相似的白蛋白 - 脂肪酸复合物，也表现出对过氧化反应的抑制作用。这些数据表明，即使与白蛋白结合，非酯化脂肪酸仍能够抑制过氧化反应，并且含有各种与其结合的脂肪酸的循环白蛋白，除了其他血浆抗氧化剂外，可能还具有一定的抗氧化作用。
