Srivastava U K, Srivastava S R, Guru P Y
Indian J Exp Biol. 1989 Jul;27(7):602-6.
A technique for obtaining isolated luteal cells without any prior enzymatic dissociation of the rat corpus luteum (CL) has been developed. With a view to obviate any kind of chemical/biophysical trauma to the cells the latter were obtained following simple migration of cells from small pieces of chopped up CL (8-10 day old) put in culture. The cells started migrating in progressively increasing numbers from these tissue pieces within 24 hr leading to monolayer formation by day 10-12 of culture. The cells were found to grow under the described conditions for 35 days without any exogenous hormonal supplementation. The technique is being utilized for characterization of different cell types of the rat CL of pregnancy and the regulatory mechanisms involved in their metabolic function and/or regression.
已开发出一种无需对大鼠黄体（CL）进行任何预先酶解就能获得分离黄体细胞的技术。为避免对细胞造成任何化学/生物物理损伤，细胞是通过将切碎的CL（8 - 10日龄）小块置于培养中后细胞简单迁移而获得的。细胞在24小时内开始从这些组织块中逐渐增多地迁移，到培养的第10 - 12天形成单层。发现这些细胞在所述条件下生长35天，无需任何外源性激素补充。该技术正用于表征妊娠大鼠CL的不同细胞类型及其代谢功能和/或退化所涉及的调节机制。
