Liu Ning, Qin Yi, Song Yuyang, Ye Dongqin, Yuan Wei, Pei Yingfang, Xue Bo, Liu Yanlin
College of Enology, Northwest A&F University, 22 Xinong Road, Yangling, 712100, Shaanxi, China.
Shaanxi Engineering Research Center for Viti-viniculture, Yangling, 712100, Shaanxi, China.
World J Microbiol Biotechnol. 2015 Nov;31(11):1781-92. doi: 10.1007/s11274-015-1929-8. Epub 2015 Sep 1.
In order to select potential indigenous Saccharomyces strains, diversity of indigenous Saccharomyces strains in Shanshan County (Xinjiang, China) was preliminarily analyzed. Twenty-one genotypes were found through interdelta fingerprinting analysis. According to this result, representatives of each genotype were chosen to test the enological criteria. After tests of fermentation characteristics and growth ability, eight strains were finally selected as starters to further fermentation of Merlot must for aroma analysis and sensory evaluation at the same testing conditions, with one commercial strain F15 as control. Each strain of Saccharomyces cerevisiae produced individual volatiles in different concentrations and combinations which significantly influenced resulting wine flavour. Except of LFP522, all indigenous isolates produced more concentration of esters than F15. Higher concentrations of linalool, β-damascenone and citral, associated with S. cerevisiae LFE1809, considerably distinguished this strain from the others. Sensory evaluation present the Merlot wine fermented by LFE1225 isolated from Merlot, had the highest sensory score.
为了筛选潜在的本土酿酒酵母菌株,对中国新疆鄯善县的本土酿酒酵母菌株多样性进行了初步分析。通过δ-间隔指纹图谱分析发现了21种基因型。根据这一结果,选择每种基因型的代表菌株来测试酿酒标准。经过发酵特性和生长能力测试后,最终选择了8株菌株作为发酵剂,在相同测试条件下对梅洛葡萄汁进行进一步发酵,用于香气分析和感官评价,以一种商业菌株F15作为对照。每株酿酒酵母产生的挥发性物质浓度和组合各不相同,这对葡萄酒的风味有显著影响。除LFP522外,所有本土分离株产生的酯类浓度均高于F15。与酿酒酵母LFE1809相关的芳樟醇、β-大马酮和柠檬醛浓度较高,使该菌株与其他菌株有显著区别。感官评价表明,从梅洛葡萄中分离出的LFE1225发酵的梅洛葡萄酒感官评分最高。