Li Hui, Zhou Fusheng, Wang Hongyan, Lin Da, Chen Gang, Zuo Xianbo, Sun Liangdan, Zhang Xuejun, Yang Sen
Department of Dermatology, Institute of Dermatology, The First Affiliated Hospital, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
Mol Med Rep. 2015 Nov;12(5):6801-6. doi: 10.3892/mmr.2015.4261. Epub 2015 Aug 28.
Myosin VI has been reported to be associated with the progression of ovarian and prostate cancer. The aim of the present study was to reveal the role of myosin VI in the proliferation of melanoma. Briefly, lentivirus‑mediated short hairpin RNA (shRNA) was designed specifically to silence myosin VI in A375 and A431 cell lines. Expression levels of myosin VI were then analyzed in the two cell lines by quantitative polymerase chain reaction and western blot analyses. Cell viability was assessed using MTT and colony formation assays. In addition, the cell cycle distribution was determined by flow cytometry. The results demonstrated that knockdown of myosin VI significantly suppressed melanoma cell viability and proliferation, and induced cell cycle arrest in G0/G1 phase. To the best of our knowledge, the present study was the first to assess the role of myosin VI in the growth of melanoma. Knowledge of the underlying mechanism of the role myosin VI in skin cancer cells may aid in the development of novel methods of melanoma diagnosis and therapy in the future.
据报道，肌球蛋白VI与卵巢癌和前列腺癌的进展有关。本研究的目的是揭示肌球蛋白VI在黑色素瘤增殖中的作用。简而言之，设计了慢病毒介导的短发夹RNA（shRNA）以特异性沉默A375和A431细胞系中的肌球蛋白VI。然后通过定量聚合酶链反应和蛋白质印迹分析来分析这两种细胞系中肌球蛋白VI的表达水平。使用MTT和集落形成试验评估细胞活力。此外，通过流式细胞术确定细胞周期分布。结果表明，敲低肌球蛋白VI可显著抑制黑色素瘤细胞活力和增殖，并诱导细胞周期停滞在G0/G1期。据我们所知，本研究是首次评估肌球蛋白VI在黑色素瘤生长中的作用。了解肌球蛋白VI在皮肤癌细胞中作用的潜在机制可能有助于未来开发新的黑色素瘤诊断和治疗方法。
