Yue Xiao-Hong, Wang Zhen, Tian Dong-Dong, Zhang Jian-Wei, Zhu Kang, Ye Qiang
Children's Hospital of Zhengzhou, Zhengzhou Institute of Pediatric Research, Zhengzhou 450053, China.
Medical College of Henan University of Science and Technology, Luoyang 471003, China.
J Chromatogr Sci. 2016 Feb;54(2):195-9. doi: 10.1093/chromsci/bmv128. Epub 2015 Aug 30.
A sensitive and rapid ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS-MS) method was developed to determine sertraline in human plasma. Sample preparation was accomplished through a simple liquid-liquid extraction with ethyl acetate. Chromatographic separation was carried out on an Acquity UPLC BEH C18 column using a gradient mobile phase system composed of acetonitrile and 1% formic acid in water at a flow rate of 0.40 mL/min. Mass spectrometric analysis was performed using a XEVO TQD mass spectrometer coupled with an electrospray ionization source in the positive ion mode. The multiple reaction monitoring transitions of m/z 306.3 → 275.2 and 326.2 → 291.1 were used to quantify for sertraline and midazolam (internal standard), respectively. The linearity of this method was found to be within the concentration range of 1.0-100.0 ng/mL with a lower limit of quantification of 1.0 ng/mL. Only 2.0 min was needed for an analytical run. This fully validated method was successfully applied to the pharmacokinetic study after an oral administration of 100 mg sertraline to 20 Chinese healthy male volunteers.
建立了一种灵敏、快速的超高效液相色谱串联质谱（UPLC-MS-MS）法测定人血浆中的舍曲林。样品制备通过用乙酸乙酯进行简单的液-液萃取完成。色谱分离在Acquity UPLC BEH C18柱上进行，使用由乙腈和1%甲酸水溶液组成的梯度流动相系统，流速为0.40 mL/min。质谱分析使用XEVO TQD质谱仪，结合电喷雾电离源在正离子模式下进行。分别使用m/z 306.3→275.2和326.2→291.1的多反应监测跃迁对舍曲林和咪达唑仑（内标）进行定量。该方法的线性范围为1.0-100.0 ng/mL，定量下限为1.0 ng/mL。一次分析运行仅需2.0分钟。该经过充分验证的方法成功应用于20名中国健康男性志愿者口服100 mg舍曲林后的药代动力学研究。
