Hurd Thomas R, Sanchez Carlos G, Teixeira Felipe K, Petzold Chris, Dancel-Manning Kristen, Wang Ju-Yu S, Lehmann Ruth, Liang Feng-Xia A
Howard Hughes Medical Institute (HHMI), New York University School of Medicine, New York, NY, 10016, USA.
Methods Mol Biol. 2015;1328:151-62. doi: 10.1007/978-1-4939-2851-4_11.
The Drosophila melanogaster ovary is a powerful, genetically tractable system through which one can elucidate the principles underlying cellular function and organogenesis in vivo. In order to understand the intricate process of oogenesis at the subcellular level, microscopic analysis with the highest possible resolution is required. In this chapter, we describe the preparation of ovaries for ultrastructural analysis using transmission electron microscopy and focused ion beam scanning electron microscopy. We discuss and provide protocols for chemical fixation of Drosophila ovaries that facilitate optimal imaging with particular attention paid to preserving and resolving mitochondrial membrane morphology and structure.
果蝇卵巢是一个强大的、具有遗传易处理性的系统，通过该系统可以阐明体内细胞功能和器官发生的基本原理。为了在亚细胞水平上理解卵子发生的复杂过程，需要进行尽可能高分辨率的显微镜分析。在本章中，我们描述了使用透射电子显微镜和聚焦离子束扫描电子显微镜对卵巢进行超微结构分析的制备方法。我们讨论并提供了果蝇卵巢化学固定的方案，这些方案有助于实现最佳成像，特别关注线粒体膜形态和结构的保存与解析。
