Ji Chenglong, Xu Hai'e, Wang Qing, Zhao Jianmin, Wu Huifeng
Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China.
Clinical Nutrition, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China.
Fish Shellfish Immunol. 2015 Nov;47(1):79-84. doi: 10.1016/j.fsi.2015.08.028. Epub 2015 Aug 29.
Inorganic arsenic is a known pollutant with two chemical forms, arsenite (As (III)) and arsenate (As (V)), in marine environment. Clam Ruditapes philippinarum is an important fishery species along the Bohai coast. In this study, the biological effects induced by the two arsenic chemical forms (arsenite and arsenate) were compared using multiple biochemical indices in the digestive glands of clam R. philippinarum. The production of reactive oxygen species, antioxidant enzyme activities and metabolic responses exhibited that both As (III) and As (V) induced immune, oxidative and osmotic stresses in clam digestive glands. The differential metabolic biomarkers, histidine and taurine, indicated the differential responsive mechanisms in osmotic regulation in clam digestive glands. In addition, both arsenic treatments enhanced the anaerobiosis metabolism in clam digestive glands. Overall, this work illustrated that arsenite and arsenate induced similar biological effects in clams, which might be accounted for the biological transformation of arsenate to arsenite in clams.
无机砷是一种已知的污染物，在海洋环境中有亚砷酸盐（As(III)）和砷酸盐（As(V)）两种化学形态。菲律宾蛤仔是渤海沿岸重要的渔业品种。在本研究中，利用菲律宾蛤仔消化腺中的多种生化指标，比较了两种砷化学形态（亚砷酸盐和砷酸盐）诱导的生物学效应。活性氧的产生、抗氧化酶活性和代谢反应表明，As(III)和As(V)均在蛤仔消化腺中诱导了免疫、氧化和渗透应激。差异代谢生物标志物组氨酸和牛磺酸表明了蛤仔消化腺渗透调节中的差异响应机制。此外，两种砷处理均增强了蛤仔消化腺中的无氧代谢。总体而言，这项工作表明亚砷酸盐和砷酸盐在蛤仔中诱导了相似的生物学效应，这可能是由于蛤仔中砷酸盐向亚砷酸盐的生物转化所致。
