Rousset Davy, Durand Thibaut
a Institut National de Recherche et de Sécurité (INRS) , Département Métrologie des Polluants , Vandoeuvre , France.
J Occup Environ Hyg. 2016;13(1):71-83. doi: 10.1080/15459624.2015.1078467.
Modification of an existing sequential extraction procedure for inorganic beryllium species in the particulate matter of emissions and in working areas is described. The speciation protocol was adapted to carry out beryllium extraction in closed-face cassette sampler to take wall deposits into account. This four-step sequential extraction procedure aims to separate beryllium salts, metal, and oxides from airborne particles for individual quantification. Characterization of the beryllium species according to their solubility in air samples may provide information relative to toxicity, which is potentially related to the different beryllium chemical forms. Beryllium salts (BeF(2), BeSO(4)), metallic beryllium (Bemet), and beryllium oxide (BeO) were first individually tested, and then tested in mixtures. Cassettes were spiked with these species and recovery rates were calculated. Quantitative analyses with matched matrix were performed using inductively coupled plasma mass spectrometry (ICP-MS). Method Detection Limits (MDLs) were calculated for the four matrices used in the different extraction steps. In all cases, the MDL was below 4.2 ng/sample. This method is appropriate for assessing occupational exposure to beryllium as the lowest recommended threshold limit values are 0.01 µg.m(-3) in France([) (1) (]) and 0.05 µg.m(-3) in the USA.([ 2 ]) The protocol was then tested on samples from French factories where occupational beryllium exposure was suspected. Beryllium solubility was variable between factories and among the same workplace between different tasks.
本文描述了一种对现有顺序萃取程序的改进方法，该程序用于分析排放颗粒物和工作区域中的无机铍物种。该形态分析方案经过调整，可在封闭式采样盒中进行铍的萃取，以考虑壁面沉积物。这种四步顺序萃取程序旨在从空气中的颗粒物中分离出铍盐、金属和氧化物，以便进行单独定量。根据铍物种在空气样本中的溶解度进行表征，可能会提供与毒性相关的信息，这可能与不同的铍化学形态有关。首先分别对铍盐（BeF₂、BeSO₄）、金属铍（Bemet）和氧化铍（BeO）进行了测试，然后对混合物进行了测试。在采样盒中加入这些物种，并计算回收率。使用电感耦合等离子体质谱法（ICP-MS）对匹配基质进行定量分析。计算了不同萃取步骤中使用的四种基质的方法检测限（MDL）。在所有情况下，MDL均低于4.2 ng/样本。该方法适用于评估职业性铍暴露，因为法国的最低推荐阈值限值为0.01 µg·m⁻³([1])，美国为0.05 µg·m⁻³([2])。然后，该方案在法国疑似存在职业性铍暴露的工厂的样本上进行了测试。不同工厂之间以及同一工作场所不同任务之间的铍溶解度存在差异。
