Chen Libin, Shi Tingting, Xia Xinyi, Miao Jingjing, Mao Chun, Huang Bin, Shen Jian
J Nanosci Nanotechnol. 2015 Jan;15(1):144-50. doi: 10.1166/jnn.2015.8772.
This development of the micro- and nanoparticle technology will bring a new momentum to drug delivery and biomedical therapy. In this case, heparin-loaded polycaprolactone microspheres (PCL-Hep MSs) were prepared by a single-step phase separation method, and characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), and fourier transform infrared spectroscopy (FTIR). The hemocompatibility and anticoagulant effect of the PCL-Hep MSs were investigated for heparin loading and release study, coagulation tests, hemolysis assay, morphological changes of red blood cells, complement activation, and cytotoxicity experiments. The results showed that the PCL-Hep MSs are hemocompatible with low level of cytotoxicity. Moreover, they have the potential to be used as a mild anticoagulant compared to pure heparin.
微米和纳米颗粒技术的这一发展将为药物递送和生物医学治疗带来新的动力。在此情况下，通过单步相分离法制备了负载肝素的聚己内酯微球（PCL-Hep MSs），并通过透射电子显微镜（TEM）、动态光散射（DLS）和傅里叶变换红外光谱（FTIR）对其进行了表征。针对肝素的负载和释放研究、凝血试验、溶血测定、红细胞形态变化、补体激活及细胞毒性实验，对PCL-Hep MSs的血液相容性和抗凝作用进行了研究。结果表明，PCL-Hep MSs具有血液相容性且细胞毒性水平较低。此外，与纯肝素相比，它们有潜力用作温和的抗凝剂。
