Yamakawa Hideaki, Hayashi Masahiro, Tanaka Kaori, Kuwano Kazuyoshi
Division of Respiratory Medicine, Department of Internal Medicine, Jikei University School of Medicine, Kashiwa Hospital, Japan.
Intern Med. 2015;54(17):2231-4. doi: 10.2169/internalmedicine.54.4950. Epub 2015 Sep 1.
We report a case of empyema due to Gemella morbillorum. In this case, an analysis of the aspirate from the pleural effusion revealed empyema and evidence of a Gram-positive coccal bacteria. A biochemical identification system labelled the bacteria as 'unclassified', although we initially suspected the bacterium to belong to the Streptococcus species. 16S ribosomal RNA (16S rRNA) gene sequencing and a phylogenetic tree analysis of the isolated strain confirmed the presence of Gemella morbillorum. To ascertain the true incidence of Gemella species in empyema, 16S rRNA gene sequencing should be used when the standard conventional biochemical methods fail to identify the organism or it identifies it with a low degree of reliability.
我们报告了一例由麻疹孪生球菌引起的脓胸病例。在此病例中，对胸腔积液抽吸物的分析显示为脓胸且有革兰氏阳性球菌的证据。尽管我们最初怀疑该细菌属于链球菌属，但生化鉴定系统将该细菌标记为“未分类”。对分离菌株进行的16S核糖体RNA（16S rRNA）基因测序和系统发育树分析证实了麻疹孪生球菌的存在。为确定孪生球菌属在脓胸中的真实发病率，当标准的传统生化方法无法鉴定该微生物或鉴定可靠性较低时，应使用16S rRNA基因测序。
