低传代登革病毒4型分离株的延迟抗体依赖性增强作用
Delayed antibody dependent enhancement of low passage dengue virus 4 isolates.
作者信息
Wikan Nitwara, Libsittikul Sirikwan, Yoksan Sutee, Auewarakul Prasert, Smith Duncan R
机构信息
Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonthol Sai 4, Salaya, Nakorn Pathom, 73170, Thailand.
Center for Emerging and Neglected Infectious Diseases, Mahidol University, Salaya Campus, 25/25 Phuttamonthol Sai 4, Salaya, Nakorn Pathom, 73170, Thailand.
出版信息
BMC Res Notes. 2015 Sep 2;8:399. doi: 10.1186/s13104-015-1381-8.
BACKGROUND
The concept of antibody dependent enhancement (ADE) of dengue virus (DENV) infection is a cornerstone of our current understanding of dengue pathogenesis, although some questions as to the mechanism remain, particularly in regards to the behavior of low and high passage virus isolates. This study utilized two low passage DENV 4 isolates and a laboratory adapted DENV 4 isolate to investigate the potential of low passage isolates to undergo ADE.
RESULTS
Little or no ADE of infection was observed on day 2 post infection with low passage isolates, while high enhancement of infection was seen with the laboratory adapted virus. However, both of the low passage isolates showed high levels of infection (60-100%) by day 5 post infection.
CONCLUSIONS
These results show that low passage DENV 4 viruses undergo ADE mediated infection, but that the process is significantly temporally delayed as compared to laboratory adapted DENV 4.
背景
登革病毒（DENV）感染的抗体依赖性增强（ADE）概念是我们目前对登革热发病机制理解的基石，尽管关于其机制仍存在一些问题，特别是关于低代和高代病毒分离株的行为。本研究利用两株低代DENV 4分离株和一株实验室适应的DENV 4分离株来研究低代分离株发生ADE的可能性。
结果
感染低代分离株后第2天未观察到感染的ADE或仅有少量ADE，而实验室适应病毒则出现了高度的感染增强。然而，两株低代分离株在感染后第5天均显示出高水平的感染（60 - 100%）。
结论
这些结果表明，低代DENV 4病毒会经历ADE介导的感染，但与实验室适应的DENV 4相比，这一过程在时间上有显著延迟。
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