尿中5-羟甲基尿嘧啶和8-氧代-7,8-二氢鸟嘌呤作为结直肠癌患者潜在生物标志物的研究
Urinary 5-hydroxymethyluracil and 8-oxo-7,8-dihydroguanine as potential biomarkers in patients with colorectal cancer.
作者信息
Rozalski Rafal, Gackowski Daniel, Siomek-Gorecka Agnieszka, Starczak Marta, Modrzejewska Martyna, Banaszkiewicz Zbigniew, Olinski Ryszard
机构信息
a Department of Clinical Biochemistry and.
出版信息
Biomarkers. 2015;20(5):287-91. doi: 10.3109/1354750X.2015.1068860. Epub 2015 Jul 31.
CONTEXT
Oxidative stress linked with chronic inflammation is associated with etiology of the colorectal cancer.
OBJECTIVES
To assess the diagnostic utility of urinary excretion of oxidatively modified DNA bases/nucleoside: 8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 5-hydroxymethyluracil (5-hmUra).
MATERIALS AND METHODS
Seventy-two healthy controls, 15 patients with adenomas and 56 colorectal cancer patients were recruited.
RESULTS
The receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) for all markers tested separately was <0.7. The combination of these modifications showed better diagnostic power (AUC = 0.778 for 8-oxoGua + 8-oxodG)/5hmUra ratio).
CONCLUSION
Urinary DNA modifications may reflect the oxidative stress/chronic inflammation in colorectal cancer but diagnostic performance for early-detection is moderate.
背景
与慢性炎症相关的氧化应激与结直肠癌的病因有关。
目的
评估氧化修饰的DNA碱基/核苷（8-氧代-7,8-二氢鸟嘌呤（8-氧代鸟嘌呤）、8-氧代-7,8-二氢-2'-脱氧鸟苷（8-氧代脱氧鸟苷）和5-羟甲基尿嘧啶（5-羟甲基尿嘧啶））尿排泄的诊断效用。
材料与方法
招募了72名健康对照者、15名腺瘤患者和56名结直肠癌患者。
结果
受试者工作特征（ROC）曲线分析表明，单独测试的所有标志物的曲线下面积（AUC）均<0.7。这些修饰的组合显示出更好的诊断能力（8-氧代鸟嘌呤+8-氧代脱氧鸟苷）/5-羟甲基尿嘧啶比值的AUC = 0.778）。
结论
尿DNA修饰可能反映结直肠癌中的氧化应激/慢性炎症，但早期检测的诊断性能中等。
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