Mallafré-Muro Celia, Llambrich Maria, Cumeras Raquel, Pardo Antonio, Brezmes Jesús, Marco Santiago, Gumà Josep
Department of Electronics and Biomedical Engineering, University of Barcelona, 08028 Barcelona, Spain.
Signal and Information Processing for Sensing Systems Group, Institute for Bioengineering of Catalonia, The Barcelona Institute of Science and Technology, 08028 Barcelona, Spain.
Cancers (Basel). 2021 May 21;13(11):2534. doi: 10.3390/cancers13112534.
To increase compliance with colorectal cancer screening programs and to reduce the recommended screening age, cheaper and easy non-invasiveness alternatives to the fecal immunochemical test should be provided. Following the PRISMA procedure of studies that evaluated the metabolome and volatilome signatures of colorectal cancer in human urine samples, an exhaustive search in PubMed, Web of Science, and Scopus found 28 studies that met the required criteria. There were no restrictions on the query for the type of study, leading to not only colorectal cancer samples versus control comparison but also polyps versus control and prospective studies of surgical effects, CRC staging and comparisons of CRC with other cancers. With this systematic review, we identified up to 244 compounds in urine samples (3 shared compounds between the volatilome and metabolome), and 10 of them were relevant in more than three articles. In the meta-analysis, nine studies met the criteria for inclusion, and the results combining the case-control and the pre-/post-surgery groups, eleven compounds were found to be relevant. Four upregulated metabolites were identified, 3-hydroxybutyric acid, L-dopa, L-histidinol, and N1, N12-diacetylspermine and seven downregulated compounds were identified, pyruvic acid, hydroquinone, tartaric acid, and hippuric acid as metabolites and butyraldehyde, ether, and 1,1,6-trimethyl-1,2-dihydronaphthalene as volatiles.
为提高对结直肠癌筛查项目的依从性并降低推荐的筛查年龄，应提供比粪便免疫化学检测更便宜且易于操作的非侵入性替代方法。按照对人类尿液样本中结直肠癌的代谢组和挥发组特征进行评估的研究的PRISMA程序，在PubMed、科学网和Scopus中进行了详尽搜索，发现有28项研究符合要求标准。对研究类型的查询没有限制，这不仅导致了结直肠癌样本与对照的比较，还包括息肉与对照以及手术效果、结直肠癌分期和结直肠癌与其他癌症比较的前瞻性研究。通过这项系统评价，我们在尿液样本中鉴定出多达244种化合物（挥发组和代谢组之间有3种共享化合物），其中10种在三篇以上文章中具有相关性。在荟萃分析中，有9项研究符合纳入标准，结合病例对照和手术前后组的结果，发现有11种化合物具有相关性。鉴定出4种上调代谢物，即3-羟基丁酸、L-多巴、L-组氨醇和N1,N12-二乙酰精胺，以及7种下调化合物，即作为代谢物的丙酮酸、对苯二酚、酒石酸和马尿酸，以及作为挥发物的丁醛、醚和1,1,6-三甲基-1,2-二氢萘。
