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利用基质辅助激光解吸/电离飞行时间质谱分析鉴定浆液性卵巢腺癌中的候选生物标志物质量（m/z）范围

Identification of candidate biomarker mass (m/z) ranges in serous ovarian adenocarcinoma using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling.

作者信息

Periyasamy Amutha, Gopisetty Gopal, Veluswami Sridevi, Joyimallaya Subramanium Malliga, Thangarajan Rajkumar

机构信息

a Department of Molecular Oncology .

出版信息

Biomarkers. 2015;20(5):292-8. doi: 10.3109/1354750X.2015.1068862.

DOI:10.3109/1354750X.2015.1068862
PMID:26329525
Abstract

OBJECTIVE

To differentiate plasma from ovarian cancer and healthy individuals using MALDI-TOF mass spectroscopy.

MATERIALS AND METHODS

MALDI-TOF was used to generate profiles of immuno-depleted plasma samples (89 cancers and 199 healthy individuals) that were fractionated using three types of magnetic beads (HIC8, WCX and IMAC-Cu).

RESULTS

Differentially expressed mass ranges showing >1.5-2-fold change in expression from HIC8 (30), WCX (12) and IMAC-Cu (6) fractions were identified. Cross validation and recognition capability scores for the models indicated discrimination between the classes.

CONCLUSIONS

Spectral profiles can differentiate plasma samples of ovarian cancer patients from healthy individuals.

摘要

目的

使用基质辅助激光解吸电离飞行时间质谱法区分卵巢癌患者与健康个体的血浆。

材料与方法

采用基质辅助激光解吸电离飞行时间质谱法生成经免疫去除的血浆样本（89例癌症患者和199例健康个体）的图谱，这些样本使用三种磁珠（疏水相互作用色谱8、弱阳离子交换和固定化金属离子亲和色谱-铜）进行了分级分离。

结果

鉴定出在疏水相互作用色谱8（30个）、弱阳离子交换（12个）和固定化金属离子亲和色谱-铜（6个）分级分离中表达变化>1.5至2倍的差异表达质量范围。模型的交叉验证和识别能力得分表明了不同类别之间的区分。

结论

光谱图谱可区分卵巢癌患者与健康个体的血浆样本。

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