Gurley Kay E, Moser Russell D, Kemp Christopher J
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109.
Cold Spring Harb Protoc. 2015 Sep 1;2015(9):pdb.prot077446. doi: 10.1101/pdb.prot077446.
In this protocol, urethane (ethyl carbamate) is used to induce lung tumors in mice. The use of urethane as an experimental carcinogen is especially attractive as it is inexpensive, relatively safe to handle, stable, and water soluble, and the protocol involves simple intraperitoneal (i.p.) injections in young mice. Urethane typically induces bronchioalveolar adenomas and, to a lesser extent, adenocarcinomas that resemble the adenocarcinoma subtype of non-small cell lung carcinoma. On a sensitive genetic background such as A/J, mice develop multiple adenomas visible on the lung surface by 25 wk, followed by the appearance of adenocarcinomas by 40 wk. Less-sensitive strains such as B6/129 develop tumors with a longer latency.
在本实验方案中，氨基甲酸乙酯（urethane，即乙胺基甲酸盐）用于诱导小鼠肺部肿瘤。使用氨基甲酸乙酯作为实验致癌物特别有吸引力，因为它价格便宜、操作相对安全、性质稳定且可溶于水，并且该实验方案只需对幼鼠进行简单的腹腔内（i.p.）注射。氨基甲酸乙酯通常会诱发支气管肺泡腺瘤，在较小程度上还会诱发类似于非小细胞肺癌腺癌亚型的腺癌。在如A/J这样敏感的遗传背景下，小鼠在25周时肺部表面会出现多个可见的腺瘤，到40周时会出现腺癌。像B6/129这样敏感性较低的品系发生肿瘤的潜伏期更长。
