Severini Francesco, Nocita Emanuela, Tosini Fabio
Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
J Med Entomol. 2015 Nov;52(6):1357-60. doi: 10.1093/jme/tjv108. Epub 2015 Aug 19.
Wound myiasis is the infestation of open wounds of mammalian hosts caused by larvae of various species of flies. This kind of myiasis can be a serious problem for immobilized patients with open wounds. Here, we identify a dipteran larva found in the tracheostomy wound of a child affected by a severe spinal muscular atrophy. The collected larva was dissected and microscopically analyzed. DNA was extracted from part of the larva and used for the molecular identification. A 487 bp fragment, including part of 5.8 S, the internal transcribed spacer 2 (ITS2), and part of 28S, was amplified using a novel PCR assay to be cloned and sequenced. The barcode region of cytochrome oxidase I (COI) was also cloned and sequenced after PCR amplification. The larva, designated as SASI1, was identified as a third instar of Sarcophaga sp. The COI sequencing confirmed a low similarity with Sarcophaga ruficornis (F.) (95%), yet COI showed a 100% similarity with Sarcophaga argyrostoma (Robineau-Desvoidy, 1830) species. Therefore, SASI1 was identified as a S. argyrostoma larva on the basis of its COI barcode. This is one of the rare cases of myiasis of tracheostomy wound and the first caused by S. argyrostoma.
创伤性蝇蛆病是由各种蝇类幼虫对哺乳动物宿主的开放性伤口进行侵袭所致。对于有开放性伤口的行动不便患者而言，这种蝇蛆病可能会成为一个严重问题。在此，我们鉴定出在一名患有严重脊髓性肌萎缩症儿童的气管造口伤口中发现的一种双翅目幼虫。对采集到的幼虫进行解剖并做显微镜分析。从幼虫的一部分提取DNA并用于分子鉴定。使用一种新型PCR检测方法扩增出一个487 bp的片段，该片段包括5.8 S的一部分、内转录间隔区2（ITS2）以及28S的一部分，随后进行克隆和测序。细胞色素氧化酶I（COI）的条形码区域在PCR扩增后也进行了克隆和测序。该幼虫被命名为SASI1，鉴定为肉蝇属的三龄幼虫。COI测序证实其与红角肉蝇（F.）相似度较低（95%），但COI显示与银口肉蝇（Robineau - Desvoidy，1830）物种相似度为100%。因此，基于其COI条形码，SASI1被鉴定为银口肉蝇幼虫。这是气管造口伤口蝇蛆病的罕见病例之一，也是首例由银口肉蝇引起的病例。
