Keyes Paul H, Rams Thomas E
formerly National Institute of Dental Research, National Institutes of Health, Bethesda, MD, USA.
Department of Periodontics, University of Pennsylvania School of Dental Medicine, Philadelphia, PA, USA.
Newsl Int Acad Periodontol. 1993 Jan;3(1-4):1-5.
The organization and behavior of spirochetes in human subgingival plaques was studied with phase-contrast microscopy. Wet-mounts of non-dispersed subgingival microbial specimens from deep pockets of 10 persons with untreated adult periodontitis revealed "brush formations" with outer coatings of closely-massed spirochetes exhibiting synchronized motility. Monolayers of closely-packed spirochetes co-aggregated with "brush formation" monofilaments were obtained by using mineral oil as a mounting medium for wet-mount preparations. Spirochetes were observed to produce collectively coordinated metachronal wave patterns with their cell movements along the outer surfaces of the "brush formation" monofilaments, rather than flexing independently and at random. Organized spirochetal activity in subgingival plaques may serve as a virulence factor contributing to the periodontopathic potential of spirochetes and/or other microbial species.
利用相差显微镜研究了人龈下菌斑中螺旋体的组织和行为。对10例未经治疗的成人牙周炎患者深牙周袋中非分散的龈下微生物标本进行湿片观察，发现有“刷状结构”，其外层为紧密聚集的螺旋体，呈现同步运动。通过使用矿物油作为湿片制备的封固介质，获得了与“刷状结构”单丝共聚集的紧密堆积螺旋体单层。观察到螺旋体沿着“刷状结构”单丝的外表面进行细胞运动，产生集体协调的相继波模式，而不是独立且随机地弯曲。龈下菌斑中有组织的螺旋体活动可能是一种毒力因子，有助于螺旋体和/或其他微生物物种的牙周致病潜能。
