基于水母发光蛋白平台筛选拟南芥中与钙信号相关的胁迫感应通道的启示。
Enlightenment on the aequorin-based platform for screening Arabidopsis stress sensory channels related to calcium signaling.
作者信息
Yu Zhiming, Taylor Jemma L, He Yue, Ni Jun
机构信息
a College of Life and Environmental Sciences ; Hangzhou Normal University ; Hangzhou , China.
b School of Life Sciences ; Gibbet Hill Campus; University of Warwick ; Coventry , United Kingdom.
出版信息
Plant Signal Behav. 2015;10(11):e1057366. doi: 10.1080/15592324.2015.1057366.
Free calcium ions (Ca(2+)) are an important signal molecule in response to a large array of external stimuli encountered by plants. Using the aequorin-based Ca(2+) recording system, tremendous progress has been made in understanding the Ca(2+) responses to biotic or abiotic stresses in dicotyledonous Arabidopsis. However, due to the lack of a similar detection system, little information has been obtained from the monocotyledonous rice (Oryza sativa). Recombinant aequorin has been introduced into rice, and the Ca(2+) responses to NaCl and H2O2 in rice roots were characterized. Although rice calcium signal sensor research has just started, the transgenic rice expressing aequorin provides a good platform to study rice adapted to different environmental conditions.
游离钙离子（Ca(2+)）是植物应对大量外部刺激时的重要信号分子。利用基于水母发光蛋白的Ca(2+)记录系统，在理解双子叶拟南芥中Ca(2+)对生物或非生物胁迫的响应方面已取得了巨大进展。然而，由于缺乏类似的检测系统，关于单子叶水稻（Oryza sativa）的相关信息知之甚少。重组水母发光蛋白已被导入水稻中，并且对水稻根中Ca(2+)对NaCl和H2O2的响应进行了表征。尽管水稻钙信号传感器的研究刚刚起步，但表达水母发光蛋白的转基因水稻为研究水稻适应不同环境条件提供了一个良好的平台。
Zotero 插件