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基于水母发光蛋白平台筛选拟南芥中与钙信号相关的胁迫感应通道的启示。

Enlightenment on the aequorin-based platform for screening Arabidopsis stress sensory channels related to calcium signaling.

作者信息

Yu Zhiming, Taylor Jemma L, He Yue, Ni Jun

机构信息

a College of Life and Environmental Sciences ; Hangzhou Normal University ; Hangzhou , China.

b School of Life Sciences ; Gibbet Hill Campus; University of Warwick ; Coventry , United Kingdom.

出版信息

Plant Signal Behav. 2015;10(11):e1057366. doi: 10.1080/15592324.2015.1057366.

DOI:10.1080/15592324.2015.1057366
PMID:26336841
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4883862/
Abstract

Free calcium ions (Ca(2+)) are an important signal molecule in response to a large array of external stimuli encountered by plants. Using the aequorin-based Ca(2+) recording system, tremendous progress has been made in understanding the Ca(2+) responses to biotic or abiotic stresses in dicotyledonous Arabidopsis. However, due to the lack of a similar detection system, little information has been obtained from the monocotyledonous rice (Oryza sativa). Recombinant aequorin has been introduced into rice, and the Ca(2+) responses to NaCl and H2O2 in rice roots were characterized. Although rice calcium signal sensor research has just started, the transgenic rice expressing aequorin provides a good platform to study rice adapted to different environmental conditions.

摘要

游离钙离子(Ca(2+))是植物应对大量外部刺激时的重要信号分子。利用基于水母发光蛋白的Ca(2+)记录系统,在理解双子叶拟南芥中Ca(2+)对生物或非生物胁迫的响应方面已取得了巨大进展。然而,由于缺乏类似的检测系统,关于单子叶水稻(Oryza sativa)的相关信息知之甚少。重组水母发光蛋白已被导入水稻中,并且对水稻根中Ca(2+)对NaCl和H2O2的响应进行了表征。尽管水稻钙信号传感器的研究刚刚起步,但表达水母发光蛋白的转基因水稻为研究水稻适应不同环境条件提供了一个良好的平台。

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本文引用的文献

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J Exp Bot. 2015 May;66(9):2535-45. doi: 10.1093/jxb/erv043. Epub 2015 Mar 9.
2
OSCA1 mediates osmotic-stress-evoked Ca2+ increases vital for osmosensing in Arabidopsis.OSCA1 介导渗透胁迫诱导的 Ca2+ 增加,对拟南芥的渗透感应至关重要。
Nature. 2014 Oct 16;514(7522):367-71. doi: 10.1038/nature13593. Epub 2014 Aug 27.
3
Toward understanding transcriptional regulatory networks in abiotic stress responses and tolerance in rice.旨在理解水稻非生物胁迫响应与耐受性中的转录调控网络。
Rice (N Y). 2012 Mar 8;5(1):6. doi: 10.1186/1939-8433-5-6. eCollection 2012.
4
DUF221 proteins are a family of osmosensitive calcium-permeable cation channels conserved across eukaryotes.DUF221蛋白是一类在真核生物中保守的渗透压敏感型钙通透性阳离子通道家族。
Cell Res. 2014 May;24(5):632-5. doi: 10.1038/cr.2014.14. Epub 2014 Feb 7.
5
Identification of a plant receptor for extracellular ATP.鉴定细胞外 ATP 的植物受体。
Science. 2014 Jan 17;343(6168):290-4. doi: 10.1126/science.343.6168.290.
6
The actin-related Protein2/3 complex regulates mitochondrial-associated calcium signaling during salt stress in Arabidopsis.拟肌动蛋白相关蛋白 2/3 复合物在盐胁迫下调节拟南芥中线粒体相关钙信号。
Plant Cell. 2013 Nov;25(11):4544-59. doi: 10.1105/tpc.113.117887. Epub 2013 Nov 26.
7
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Plant Cell. 2013 Jun;25(6):2115-31. doi: 10.1105/tpc.113.113068. Epub 2013 Jun 25.
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