Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", 660036 Krasnoyarsk, Russia.
School of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.
Int J Mol Sci. 2020 Oct 10;21(20):7465. doi: 10.3390/ijms21207465.
The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate-coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization-Ca-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme-substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases.
在大多数已知的海洋生物中，生物发光系统的功能都是基于同一底物——腔肠素（CTZ）的氧化反应，由荧光素酶催化。尽管这些酶的结构和作用机制存在多样性，但它们可以被归为一类共同的 CTZ 依赖性荧光酶。在这些酶中，有两种截然不同的系统组织类型——钙调节光蛋白和荧光酶本身，它们根据经典的酶底物动力学原理发挥作用。随着对这些系统的深入和全面的基础研究，已经开发出了将其作为分析中的高灵敏度报告器实际应用的方法和技术。旨在创造具有新独特性质的人工荧光酶和合成 CTZ 类似物的研究，导致了基于它们的新实验分析方法的发展。对于首次使用者来说，许多基于 CTZ 依赖性荧光酶的即用型检测系统的商业可用性也很重要。基于这些生物发光系统的分析方法的发展目前正在蓬勃发展。所考虑的生物发光系统已成功应用于各个生物研究领域，这证明了它们是一种强大的分析工具。在本文中，我们考虑了涉及这些荧光酶的主要方向、结果和成就。
