Kakpakova E S, Malakhova E M, Gnedoĭ S N, Pochechueva T V, Filatov I A, Dudkin S M, Stavrovskaia A A
Biull Eksp Biol Med. 1989 Nov;108(11):618-20.
Five Hybridoma clones producing monoclonal antibodies (MAT) to bovine nerve growth factor (NGF) were developed. The biological effects of antibodies were studied: the influence of MAT on neurit outgrowth induced by NGF in rat pheochromocytoma PC12 or spinal chicken ganglia was investigated. MAT fell into two groups. Two of them inhibited neurit induction by NGF, three others stimulated this process. The stimulation of the neurit outgrowth by MAT was observed at low concentration of NGF (3 ng/ml of culture medium). Mechanisms of antibodies effects are discussed.
已获得五个产生抗牛神经生长因子（NGF）单克隆抗体（MAT）的杂交瘤克隆。研究了这些抗体的生物学效应：研究了MAT对NGF诱导大鼠嗜铬细胞瘤PC12或鸡脊髓神经节神经突生长的影响。MAT分为两组。其中两个抑制NGF诱导的神经突生长，另外三个则刺激这一过程。在低浓度NGF（3 ng/ml培养基）下观察到MAT对神经突生长的刺激作用。文中讨论了抗体作用的机制。
