Nowacka-Zawisza Maria, Wiśnik Ewelina, Wasilewski Andrzej, Skowrońska Milena, Forma Ewa, Bryś Magdalena, Różański Waldemar, Krajewska Wanda M
Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland.
Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland ; Department of Biophysics of Environmental Pollution, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland.
Anal Cell Pathol (Amst). 2015;2015:828646. doi: 10.1155/2015/828646. Epub 2015 Aug 3.
Genetic polymorphisms in DNA repair genes may induce individual variations in DNA repair capacity, which may in turn contribute to the risk of cancer developing. Homologous recombination repair (HRR) plays a critical role in maintaining chromosomal integrity and protecting against carcinogenic factors. The aim of the present study was to evaluate the relationship between prostate cancer risk and the presence of single nucleotide polymorphisms (SNPs) in the genes involved in HRR, that is, RAD51 (rs1801320 and rs1801321), RAD51B (rs10483813 and rs3784099), XRCC2 (rs3218536), and XRCC3 (rs861539). Polymorphisms were analyzed by PCR-RFLP and Real-Time PCR in 101 patients with prostate adenocarcinoma and 216 age- and sex-matched controls. A significant relationship was detected between the RAD51 gene rs1801320 polymorphism and increased prostate cancer risk. Our results indicate that the RAD51 gene rs1801320 polymorphism may contribute to prostate cancer susceptibility in Poland.
DNA修复基因中的遗传多态性可能会导致DNA修复能力的个体差异，进而可能增加患癌风险。同源重组修复（HRR）在维持染色体完整性和抵御致癌因素方面起着关键作用。本研究的目的是评估前列腺癌风险与参与HRR的基因（即RAD51（rs1801320和rs1801321）、RAD51B（rs10483813和rs3784099）、XRCC2（rs3218536）和XRCC3（rs861539））中单核苷酸多态性（SNP）的存在之间的关系。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和实时聚合酶链反应（Real-Time PCR）对101例前列腺腺癌患者和216例年龄及性别匹配的对照进行多态性分析。检测到RAD51基因rs1801320多态性与前列腺癌风险增加之间存在显著关系。我们的结果表明，RAD51基因rs1801320多态性可能与波兰人群的前列腺癌易感性有关。
