Zheng Qianli, Gao Shuhong
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People's Republic of China.
Biotechnol Appl Biochem. 2016 Nov;63(6):895-900. doi: 10.1002/bab.1443. Epub 2015 Nov 17.
Soybean oil is an important carbon source in kitasamycin fermentation by Streptomyces kitasatoensis. In this study, three different surfactants, Tween 80, Tween 85, and sodium dodecyl sulfate (SDS), were added in the fermentation medium to improve soybean oil utilization. Results indicated that all of these surfactants promote kitasamycin biosynthesis. When 0.5 g/L SDS was added at the beginning of fermentation, kitasamycin production increased by 55% and A content improved by 12%, compared with the control treatment (i.e., no surfactant added). Oil consumption rate and lipase activity were also improved in the presence of SDS, producing more organic acids benefiting kitasamycin biosynthesis. High butyric acid concentration in the fermentation medium containing SDS repressed C-3 acetylation and promoted A component accumulation. Additionally, utilization of oil components by S. kitasatoensis was altered. Specifically, linoleic acid was primarily used in the fermentation process with SDS, whereas oleic acid was primarily used in the fermentation process where no surfactant had been added.
大豆油是北里链霉菌发酵生产吉他霉素的重要碳源。在本研究中，在发酵培养基中添加了三种不同的表面活性剂，吐温80、吐温85和十二烷基硫酸钠（SDS），以提高大豆油的利用率。结果表明，所有这些表面活性剂均促进吉他霉素的生物合成。与对照处理（即不添加表面活性剂）相比，在发酵开始时添加0.5 g/L SDS，吉他霉素产量提高了55%，A组分含量提高了12%。在SDS存在下，油脂消耗率和脂肪酶活性也得到提高，产生了更多有利于吉他霉素生物合成的有机酸。含有SDS的发酵培养基中高浓度的丁酸抑制了C-3乙酰化并促进了A组分的积累。此外，北里链霉菌对油脂成分的利用发生了改变。具体而言，在添加SDS的发酵过程中主要利用亚油酸，而在未添加表面活性剂的发酵过程中主要利用油酸。
