Alrefaei Ghadeer I, Ayuob Nasra N, Ali Soad S, Al-Karim Saleh
Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia Histology and Cytology Department, Mansoura University, Mansoura, Egypt.
Folia Histochem Cytobiol. 2015;53(3):259-71. doi: 10.5603/FHC.a2015.0022. Epub 2015 Sep 4.
Although the human umbilical cord (UC) has been previously considered a medical waste, its use as a main source of fetal stem cells for regenerative medicine applications has increased over the past few years. The aim of the study was to assess the impact of the maternal age on the expression of mesenchymal stem cells (MSC) markers CD105 and CD29 in the different areas of human UC.
In this comparative cross sectional study, one hundred term UCs from five maternal age groups (20-45 years) were collected after delivery from healthy mothers and were processed to assess both immuno- and gene expression of CD105 and CD29 surface antigen markers using immunohistochemical and RT-PCR techniques.
The immunoexpression of CD105 and CD29 in the amniotic membrane (AM) and Wharton's jelly (WJ), the umbilical artery (UA) and the umbilical vein (UV) showed significant negative correlation with the maternal age (p < 0.001). Reduced amount of cells as well as the studied MSC markers and their gene expression levels were documented in older age mothers. CD105-positive MSCs were more abundant in the UA, whereas CD29-positive MSCs were more abundant in the AM and WJ.
The decreased expression of CD105 and CD29 MSCs markers with age suggests that selective isolation of MSCs from Wharton's jelly, umbilical artery or umbilical vein of younger mothers should be recommended.
尽管人脐带（UC）以前被视为医疗废物，但在过去几年中，其作为再生医学应用中胎儿干细胞的主要来源的用途有所增加。本研究的目的是评估母亲年龄对人脐带不同区域间充质干细胞（MSC）标志物CD105和CD29表达的影响。
在这项比较性横断面研究中，从健康母亲分娩后收集了来自五个母亲年龄组（20 - 45岁）的100条足月脐带，并进行处理，使用免疫组织化学和逆转录 - 聚合酶链反应（RT - PCR）技术评估CD105和CD29表面抗原标志物的免疫表达和基因表达。
羊膜（AM）、华通胶（WJ）、脐动脉（UA）和脐静脉（UV）中CD105和CD29的免疫表达与母亲年龄呈显著负相关（p < 0.001）。年龄较大的母亲中细胞数量以及所研究的MSC标志物及其基因表达水平均有所降低。CD105阳性的MSC在脐动脉中更为丰富，而CD29阳性的MSC在羊膜和华通胶中更为丰富。
随着年龄增长，CD105和CD29 MSC标志物表达降低，这表明推荐从年轻母亲的华通胶、脐动脉或脐静脉中选择性分离MSC。
