Williams James A
Nature Technology Corporation/Suite 103, 4701 Innovation Drive, Lincoln, NE 68521, USA.
Vaccines (Basel). 2013 Jun 25;1(3):225-49. doi: 10.3390/vaccines1030225.
DNA vaccination is a disruptive technology that offers the promise of a new rapidly deployed vaccination platform to treat human and animal disease with gene-based materials. Innovations such as electroporation, needle free jet delivery and lipid-based carriers increase transgene expression and immunogenicity through more effective gene delivery. This review summarizes complementary vector design innovations that, when combined with leading delivery platforms, further enhance DNA vaccine performance. These next generation vectors also address potential safety issues such as antibiotic selection, and increase plasmid manufacturing quality and yield in exemplary fermentation production processes. Application of optimized constructs in combination with improved delivery platforms tangibly improves the prospect of successful application of DNA vaccination as prophylactic vaccines for diverse human infectious disease targets or as therapeutic vaccines for cancer and allergy.
DNA疫苗接种是一项颠覆性技术，有望提供一个新的快速部署的疫苗接种平台，用基于基因的材料治疗人类和动物疾病。诸如电穿孔、无针喷射递送和脂质载体等创新技术，通过更有效的基因递送提高了转基因表达和免疫原性。本综述总结了互补载体设计创新，这些创新与领先的递送平台相结合时，能进一步提高DNA疫苗的性能。这些下一代载体还解决了诸如抗生素选择等潜在安全问题，并在典型的发酵生产过程中提高了质粒的制造质量和产量。将优化构建体与改进的递送平台相结合应用，切实改善了DNA疫苗接种作为针对多种人类传染病靶点的预防性疫苗或作为癌症和过敏治疗性疫苗成功应用的前景。
