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古代后生动物大分子复合物全景图。

Panorama of ancient metazoan macromolecular complexes.

作者信息

Wan Cuihong, Borgeson Blake, Phanse Sadhna, Tu Fan, Drew Kevin, Clark Greg, Xiong Xuejian, Kagan Olga, Kwan Julian, Bezginov Alexandr, Chessman Kyle, Pal Swati, Cromar Graham, Papoulas Ophelia, Ni Zuyao, Boutz Daniel R, Stoilova Snejana, Havugimana Pierre C, Guo Xinghua, Malty Ramy H, Sarov Mihail, Greenblatt Jack, Babu Mohan, Derry W Brent, Tillier Elisabeth R, Wallingford John B, Parkinson John, Marcotte Edward M, Emili Andrew

机构信息

Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.

Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.

出版信息

Nature. 2015 Sep 17;525(7569):339-44. doi: 10.1038/nature14877. Epub 2015 Sep 7.

DOI:10.1038/nature14877
PMID:26344197
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5036527/
Abstract

Macromolecular complexes are essential to conserved biological processes, but their prevalence across animals is unclear. By combining extensive biochemical fractionation with quantitative mass spectrometry, here we directly examined the composition of soluble multiprotein complexes among diverse metazoan models. Using an integrative approach, we generated a draft conservation map consisting of more than one million putative high-confidence co-complex interactions for species with fully sequenced genomes that encompasses functional modules present broadly across all extant animals. Clustering reveals a spectrum of conservation, ranging from ancient eukaryotic assemblies that have probably served cellular housekeeping roles for at least one billion years, ancestral complexes that have accrued contemporary components, and rarer metazoan innovations linked to multicellularity. We validated these projections by independent co-fractionation experiments in evolutionarily distant species, affinity purification and functional analyses. The comprehensiveness, centrality and modularity of these reconstructed interactomes reflect their fundamental mechanistic importance and adaptive value to animal cell systems.

摘要

大分子复合物对于保守的生物过程至关重要，但其在动物中的普遍程度尚不清楚。通过将广泛的生化分级分离与定量质谱相结合，我们在此直接研究了多种后生动物模型中可溶性多蛋白复合物的组成。采用综合方法，我们为具有全基因组测序的物种生成了一份保守图谱草案，其中包含超过一百万个假定的高可信度共复合物相互作用，涵盖了广泛存在于所有现存动物中的功能模块。聚类分析揭示了一系列保守程度，从可能至少在十亿年里一直发挥细胞管家作用的古老真核生物组装体，到积累了当代成分的祖先复合物，以及与多细胞性相关的罕见后生动物创新。我们通过在进化关系较远的物种中进行独立的共分级分离实验、亲和纯化和功能分析，验证了这些预测。这些重建的相互作用组的全面性、中心性和模块化反映了它们对动物细胞系统的基本机制重要性和适应性价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/1ecf7f65794d/nihms704400f5.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/0a557e0c3ece/nihms704400f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/f39dea27f374/nihms704400f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/3c23c09ba042/nihms704400f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/8b42b03c04e9/nihms704400f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/b1999291e613/nihms704400f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/3b5ddbae27b2/nihms704400f12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/0b75bdc37cc9/nihms704400f13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/d905362162bb/nihms704400f14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/47d9ca5d7a09/nihms704400f15.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/bd2f21568f34/nihms704400f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/5263438c3270/nihms704400f2.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96cc/5036527/1ecf7f65794d/nihms704400f5.jpg

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