使用粪便上清液抗原对犬细粒棘球绦虫感染进行血清学诊断。
Sero-diagnosis of Echinococcus granulosus infection in dogs using faecal supernatant antigen.
作者信息
Ananda K J, D'Souza Placid E, Suryanarayana V V S
机构信息
Department of Veterinary Parasitology, Veterinary College, Shimoga, 577204 India.
Department of Veterinary Parasitology, Veterinary College, Hebbal, Bangalore, 560024 India.
出版信息
J Parasit Dis. 2015 Sep;39(3):461-4. doi: 10.1007/s12639-013-0372-8. Epub 2013 Dec 1.
In the present study, sero-diagnosis of Echinococcus granulosus infection in dogs was studied using 250 field serum samples, collected from stray dogs. The three serological techniques viz., latex agglutination test (LAT), Dot-ELISA and enzyme immunotransfer blot (EITB) were used to detect E. granulosus specific anti-bodies using fecal supernatant antigen. The LAT detected positivity in 53 (21.2 %) serum samples with the sensitivity and specificity of 100 and 78.80 % respectively. Whereas, the Dot-ELISA, showed positivity in 45 (18.0 %) samples and the sensitivity and specificity were 100 and 82.0 % respectively. The specific antibodies were detected in 47 (18.80 %) serum samples by EITB and the polypeptides of 45 and 34 kDa were identified in all the positive serum samples. The sensitivity and specificity of EITB were found to be 100 and 86.80 %, respectively.
在本研究中，使用从流浪狗采集的250份野外血清样本对犬细粒棘球绦虫感染进行血清学诊断。采用乳胶凝集试验（LAT）、斑点酶联免疫吸附测定（Dot-ELISA）和酶免疫转移印迹法（EITB）这三种血清学技术，利用粪便上清液抗原检测细粒棘球绦虫特异性抗体。LAT在53份（21.2%）血清样本中检测到阳性，敏感性和特异性分别为100%和78.80%。而Dot-ELISA在45份（18.0%）样本中显示阳性，敏感性和特异性分别为100%和82.0%。EITB在47份（18.80%）血清样本中检测到特异性抗体，并且在所有阳性血清样本中鉴定出45 kDa和34 kDa的多肽。EITB的敏感性和特异性分别为100%和86.80%。
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