原头节的冷冻保存
Cryopreservation of Protoscoleces.
作者信息
Badirzadeh Alireza, Raeghi Saber, Fallah-Omrani Vahid, Rouhani Soheila
机构信息
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.
出版信息
Iran J Public Health. 2020 Jan;49(1):181-185.
BACKGROUND
The main objective of the current study was to investigate on the cryopreservation of protoscoleces of , a causative agent of cystic hydatidosis in man.
METHODS
This study was conducted on isolated protoscoleces from hydatid cysts infected livers collected from slaughterhouse of Tehran, Iran in 2016. Viability of protoscoleces was evaluated by dye test. Cryopreservation of isolated protoscoleces in the presence of Dimethylsulfoxide (DMSO) and glycerol using a three-step cooling protocol involving an initial period at -20 °C, -80 °C and liquid nitrogen was performed.
RESULTS
The mean viability rate of 10% DMSO and 15% glycerol were 9% and 8% respectively. The protoscoleces of have been successfully thawed and recovered after 6 months storage in liquid nitrogen.
CONCLUSION
Cryopreservation method needs to be improved for each species of helminthes and can be useful for other immunological and laboratorial studies.
背景
本研究的主要目的是调查人体囊性包虫病病原体的原头节的冷冻保存情况。
方法
本研究使用的原头节来自2016年从伊朗德黑兰屠宰场收集的感染包虫囊肿的肝脏。通过染色试验评估原头节的活力。采用三步冷却方案，在二甲基亚砜（DMSO）和甘油存在的情况下，将分离出的原头节在-20°C、-80°C和液氮中进行冷冻保存。
结果
10% DMSO和15%甘油处理后的原头节平均活力率分别为9%和8%。在液氮中储存6个月后，已成功解冻并复苏。
结论
冷冻保存方法需要针对每种蠕虫进行改进，并且可用于其他免疫学和实验室研究。
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