Ahram Canadian University, Faculty of Pharmacy, Pharmaceutical Chemistry Department, 6th of October City, Giza, Egypt; The American University in Cairo, School of Sciences and Engineering, Department of Chemistry, P.O. Box 74, New Cairo 11835, Egypt.
The American University in Cairo, School of Sciences and Engineering, Department of Chemistry, P.O. Box 74, New Cairo 11835, Egypt.
Anal Chim Acta. 2015 Aug 26;890:21-43. doi: 10.1016/j.aca.2015.06.038. Epub 2015 Aug 7.
Residues of antibiotics (ABs) in the aquatic environment and in food of animal origin represent a major concern, as prolonged exposure to ABs is a serious health hazard, related to both the side effects of prolonged use and the risk of developing bacterial resistance to various ABs. Given the low levels of the AB residues in complex matrices, the development of sensitive analytical methods represents a major challenge. This is certainly true for the aminoglycoside ABs (AGs) which lack a chromophore and show poor chromatographic properties in reversed-phase liquid chromatography. This paper reviews the current state of the art in the determination of AGs. Attention is paid to extraction, sample clean-up, chromatographic separation, and detection of AGs in both environmental and food samples and in plasma and serum. A general workflow for the analysis of AGs is presented which takes into account the matrix and required level of information.
抗生素（ABs）在水环境和动物源性食品中的残留是一个主要关注点，因为长期接触 ABs 是一个严重的健康危害，这既与长期使用的副作用有关，也与细菌对各种 ABs 产生耐药性的风险有关。鉴于复杂基质中 AB 残留水平较低，开发灵敏的分析方法是一个主要挑战。对于缺乏发色团且在反相液相色谱中表现出较差色谱性能的氨基糖苷类抗生素（AGs）来说，情况更是如此。本文综述了 AGs 测定的最新技术。本文关注了 AGs 在环境和食品样品以及血浆和血清中的提取、样品净化、色谱分离和检测。提出了一个分析 AGs 的通用工作流程，该流程考虑了基质和所需信息的水平。
