Yang Sung Jae, Jo Hyoung, Kim Kyung-A, Ahn Hong Ryul, Kang Suk Woo, Jung Sang Hoon
1 Department of Ophthalmology, University of Ulsan , Gangneung Asan Hospital, Gangneung, Gangwon-do, Korea.
2 Natural Product Research Center, Korea Institute of Science and Technology (KIST) , Gangneung, Gangwon-do, Korea.
J Med Food. 2016 Jan;19(1):106-9. doi: 10.1089/jmf.2014.3404. Epub 2015 Sep 8.
The purpose of this study was to evaluate the effect of ethanol extract of Diospyros kaki (EEDK) leaves on corneal neovascularization (CoNV) in rats. One week after the alkali burns in the corneas, the CoNV area coverage in the CoNV-positive control group, 100 mg/kg EEDK group, and 200 mg/kg EEDK group was 43.3% ± 5.5%, 337.7% ± 2.5%, and 27.2% ± 4.3%, respectively. The areas of CoNV in the EEDK-treated groups were significantly different from those of the CoNV group. EEDK significantly attenuated the upregulation of vascular endothelial growth factor, fibroblast growth factor, interleukin-6, and matrix metalloproteinase-2 (MMP-2) protein levels. Orally administrated D. kaki inhibited CoNV development in rats.
本研究旨在评估柿叶乙醇提取物（EEDK）对大鼠角膜新生血管化（CoNV）的影响。在角膜碱烧伤一周后，CoNV阳性对照组、100mg/kg EEDK组和200mg/kg EEDK组的CoNV面积覆盖率分别为43.3%±5.5%、337.7%±2.5%和27.2%±4.3%。EEDK治疗组的CoNV面积与CoNV组有显著差异。EEDK显著减弱了血管内皮生长因子、成纤维细胞生长因子、白细胞介素-6和基质金属蛋白酶-2（MMP-2）蛋白水平的上调。口服柿叶可抑制大鼠CoNV的发展。
