Peirone S M, Amedeo M R, Filogamo G
Arch Ital Anat Embriol. 1989 Jul-Sep;94(3):289-99.
Somites from 9H. H. chick embryos and PC12 cells were co-cultivated in synthetic medium containing N.G.F., which induces the transformation of PC12 cells into neuron-like cells. During the first two days of culture, PC12 cells retained the spherical shape and tended to cluster. Somitic mesoderm cells exhibited a fibroblastic aspect. By the third day, PC12 cells extended long processes resembling nerve fibres which surrounded and penetrated the mesodermic explants. On the 10th day of culture, contractions, limited at first to a few cells were perceptible. Later, the contractions involved large cellular masses. Microscopic observations at 10 days revealed the presence of an increasing number of fusiform mononucleated cells. Later, long and narrow multinucleated elements appeared. Such elements never developed from cultures of only somites. Immunohistochemical observations revealed a desmin positivity in both mononucleated and multinucleated elements characterizing them as myogenic cells that are formed in and because of the presence of PC12 cells which were transformed by N.G.F. into nerve cells. After 10 days of culture, PC12 cells positive to antiserum antidesmin were noted. Desmin positivity of PC12 cells leads to the conclusion that newly-formed muscle cells exert an induction on Pheocromocytoma cells which, as derivatives of the neural crest, have a greater multipotentiality.
将9日龄鸡胚的体节与PC12细胞在含有神经生长因子（N.G.F.）的合成培养基中共同培养，神经生长因子可诱导PC12细胞转化为神经元样细胞。在培养的前两天，PC12细胞保持球形并倾向于聚集。体节中胚层细胞呈现出成纤维细胞样外观。到第三天，PC12细胞伸出类似神经纤维的长突起，这些突起围绕并穿透中胚层外植体。在培养的第10天，可以察觉到起初局限于少数细胞的收缩。后来，收缩涉及大量细胞团。培养10天时的显微镜观察显示梭形单核细胞数量不断增加。后来，出现了长而窄的多核细胞成分。仅体节培养物中从未形成这样的成分。免疫组织化学观察显示，单核和多核成分中结蛋白呈阳性，表明它们是肌源性细胞，是在被N.G.F.转化为神经细胞的PC12细胞存在的情况下并因这种存在而形成的。培养10天后，观察到PC12细胞对抗结蛋白抗血清呈阳性。PC12细胞的结蛋白阳性得出这样的结论：新形成的肌肉细胞对作为神经嵴衍生物且具有更大多能性的嗜铬细胞瘤细胞施加诱导作用。
