Echevarría S, Cajigas J, Rodríguez de Lope C, Lozano J L, Pons Romero F
Gastroenterology Unit, National Hospital Marqués de Valdecilla, Faculty of Medicine, Santander, Spain.
Allergol Immunopathol (Madr). 1989 Nov-Dec;17(6):317-21.
We analyzed the response to phytohemagglutinin (PHA) of peripheral blood (PBL), intraepithelial (IEL) and lamina propia (LPL) lymphocytes in eight patients with Crohn's disease (CD). We also studied the effect of indomethacin, a prostaglandin synthetase inhibitor, and monocyte depletion on mitogen-induced stimulation of these cells. The blastic stimulation index was significantly lower in PBL and intestinal mucosa lymphocytes of CD than in controls (p less than 0.001). This index increased after addition of indomethacin in PBL and LPL and after monocyte depletion in PBL. The suppressor index on blastic stimulation, obtained when indomethacin had been added to the cultures was higher in PBL and intestinal mucosa lymphocytes of patients with CD than in controls. After monocyte depletion, this suppressor index decreased significantly in PBL and did not change in controls. These data suggest that prostaglandins could be one of the responsible factors for hyporesponsiveness of lymphocytes in CD.
我们分析了8例克罗恩病（CD）患者外周血（PBL）、上皮内（IEL）和固有层（LPL）淋巴细胞对植物血凝素（PHA）的反应。我们还研究了前列腺素合成酶抑制剂吲哚美辛和单核细胞清除对这些细胞有丝分裂原诱导刺激的影响。CD患者的PBL和肠黏膜淋巴细胞的增殖刺激指数显著低于对照组（p<0.001）。在PBL和LPL中添加吲哚美辛后以及PBL中单核细胞清除后，该指数升高。在培养物中添加吲哚美辛后获得的增殖刺激抑制指数在CD患者的PBL和肠黏膜淋巴细胞中高于对照组。单核细胞清除后，PBL中的这种抑制指数显著降低，而对照组中则无变化。这些数据表明，前列腺素可能是CD中淋巴细胞低反应性的原因之一。
