Chen G P
Zhonghua Bing Li Xue Za Zhi. 1989 Sep;18(3):217-20.
Trypan blue(T. blue) was used to block macrophages (M phi) for investigating the role of M phi in tumor-suppressing activity of polyactin A, a mannose-peptide immunomodulator extracted from alpha-streptococcus. Results showed that polyactin A could significantly inhibit the growth of transplanted S 180 tumor in mice. Anyhow, when M phi activities were blocked by T. blue, the protective effect of polyactin A was almost completely lost. Moreover, T. blue-treated mice showed larger tumor size than the controls. In addition, marked hemorrhagic necrosis was seen in the polyactin A-treated tumor, and the area of necrosis was larger than that in the controls. No significant difference was seen between T. blue-treated group and the controls. Furthermore, more M type ANAE+ cells were seen in the peripheral area of the tumors in polyactin A-treated groups than in any other group. All these findings suggest that the in vivo antitumor effect of polyactin A is produced mainly through M phi mechanisms.
锥虫蓝（T. 蓝）被用于阻断巨噬细胞（M phi），以研究M phi在多抗肌动蛋白A（一种从α-链球菌中提取的甘露糖肽免疫调节剂）的肿瘤抑制活性中的作用。结果显示，多抗肌动蛋白A可显著抑制小鼠移植性S 180肿瘤的生长。无论如何，当M phi活性被锥虫蓝阻断时，多抗肌动蛋白A的保护作用几乎完全丧失。此外，经锥虫蓝处理的小鼠肿瘤尺寸比对照组更大。另外，在多抗肌动蛋白A处理的肿瘤中可见明显的出血性坏死，坏死面积比对照组更大。锥虫蓝处理组与对照组之间未见显著差异。此外，多抗肌动蛋白A处理组肿瘤外周区域可见的M型ANAE+细胞比其他任何组都多。所有这些发现表明，多抗肌动蛋白A的体内抗肿瘤作用主要通过M phi机制产生。
