Zhang J R, Zhang X Y, Chen X T
Zhonghua Nei Ke Za Zhi. 1989 Dec;28(12):729-30, 768.
A new group of gastric cancer associated antigens (MG-Ag) in ascitic fluid and pleural effusion was detected. First of all, the monoclonal antibodies (MG series) were purified and coupled to the miniglobules. The samples to be examined were then mixed with the MG-miniglobules to react. After being blocked by serum of normal mouse, the MG-miniglobules were mixed and made to react with the monoclonal antibodies labeled with HRP. With ELISA method, the MG-Ag levels in ascitic and pleural fluid were determined in 171 patients. The mean value of 87 non-malignant patients plus 3 standard deviations was arbitrarily set as the highest normal limit. Values above this limit were found in 39 (75%) of 52 patients with lung cancer and 20 (83%) of 24 patients with gastric cancer. The MG-Ag level was elevated in 4 (4.6%) of 87 patients with benign diseases. It is not elevated in 8 patients with ovarian cancer. It is suggested that determination of MG-Ag in ascitic and pleural fluid can be used for ascertaining the nature of ascites and pleural effusion.
在腹水和胸腔积液中检测到一组新的胃癌相关抗原（MG-Ag）。首先，纯化单克隆抗体（MG系列）并将其偶联到微球上。然后将待检测样本与MG-微球混合进行反应。用正常小鼠血清封闭后，将MG-微球混合并使其与用辣根过氧化物酶（HRP）标记的单克隆抗体反应。采用酶联免疫吸附测定（ELISA）法测定了171例患者腹水和胸腔积液中的MG-Ag水平。将87例非恶性患者的平均值加上3个标准差任意设定为正常上限。52例肺癌患者中有39例（75%）、24例胃癌患者中有20例（83%）的值高于此限。87例良性疾病患者中有4例（4.6%）MG-Ag水平升高。8例卵巢癌患者中未升高。提示检测腹水和胸腔积液中的MG-Ag可用于确定腹水和胸腔积液的性质。
