酿酒酵母中的絮凝作用由RNA/DNA解旋酶Sen1p调控。
Flocculation in Saccharomyces cerevisiae is regulated by RNA/DNA helicase Sen1p.
作者信息
Singh Vikash, Azad Gajendra Kumar, Sariki Santhosh Kumar, Tomar Raghuvir S
机构信息
Laboratory of Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal 462023, India.
Laboratory of Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal 462023, India.
出版信息
FEBS Lett. 2015 Oct 7;589(20 Pt B):3165-74. doi: 10.1016/j.febslet.2015.09.006. Epub 2015 Sep 10.
The Nrd1-Nab3-Sen1 (NNS) complex terminates transcription of non-coding RNA genes and mediates degradation of the produced transcript by the nuclear exosome. The NNS complex also represses some stress response genes, by stimulating premature termination. A well-characterized stress response in yeast is flocculation, where cells aggregate to form flocs under expression of lectin-encoding genes designated as FLOs. In this study, we demonstrated the role of the NNS complex and Rrp6p in the expression of flocculation genes: FLO1, FLO5, FLO9, and FLO10. Furthermore, a deletion mutant of the RNA processing machinery (RNT1), and SEN1 mutants that are unable to interact with Rnt1p, exhibit a flocculation phenotype. In summary, we have identified a cooperative role of Rnt1p, Rrp6p and the NNS complex in the repression of FLO genes.
Nrd1-Nab3-Sen1（NNS）复合物可终止非编码RNA基因的转录，并通过核外泌体介导所产生转录本的降解。NNS复合物还通过刺激过早终止来抑制一些应激反应基因。酵母中一种特征明确的应激反应是絮凝，即细胞在称为FLOs的凝集素编码基因表达时聚集形成絮状物。在本研究中，我们证明了NNS复合物和Rrp6p在絮凝基因FLO1、FLO5、FLO9和FLO10表达中的作用。此外，RNA加工机制（RNT1）的缺失突变体以及无法与Rnt1p相互作用的SEN1突变体表现出絮凝表型。总之，我们确定了Rnt1p、Rrp6p和NNS复合物在抑制FLO基因中的协同作用。
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