Guney Tekin, Alisik Murat, Alkan Afra, Basturk Abdulkadir, Akinci Sema, Hacibekiroglu Tuba, Dilek Imdat, Erel Ozcan
a Department of Hematology , Turkiye Yuksek Ihtisas Training and Research Hospital , Ankara , Turkey.
b Department of Medical Biochemistry , Ataturk Training and Research Hospital , Ankara , Turkey.
Redox Rep. 2016 Jan;21(1):1-5. doi: 10.1179/1351000215Y.0000000042. Epub 2016 Mar 3.
The purpose of this study was to investigate paraoxonase (PON) and arylesterase (ARES) enzyme activity in adults with vitamin B12 deficiency, and specific changes in the activities of these enzymes following vitamin B12 treatment.
A total of 46 patients with vitamin B12 deficiency (aged 18-82 years) and 45 healthy volunteer controls (aged 19-64 years) participated in this study. Venous blood samples were collected, and serum vitamin B12, homocysteine (HCY), methylmalonic acid, PON1, and ARES levels were measured.
Paired comparison showed that pre- and post-treatment values for PON and ARES were similar between patients and controls (both P > 0.05). There was no statistically significant relationship between patients' pre-/post-treatment PON or HCY levels and serum vitamin B12 levels, compared with those of the control group (P > 0.05).
The results of the present study do not support the hypothesis that the antioxidant enzymes PON and ARES have an underlying role in vitamin B12 deficiency and related hyperhomocysteinemia. Our findings suggest that PON and ARES do not play a role in the systemic effects of vitamin B12 deficiency.
本研究旨在调查维生素B12缺乏的成年人的对氧磷酶(PON)和芳基酯酶(ARES)的酶活性,以及维生素B12治疗后这些酶活性的具体变化。
共有46例维生素B12缺乏患者(年龄18 - 82岁)和45名健康志愿者对照(年龄19 - 64岁)参与了本研究。采集静脉血样本,检测血清维生素B12、同型半胱氨酸(HCY)、甲基丙二酸、PON1和ARES水平。
配对比较显示,患者和对照组的PON和ARES治疗前后的值相似(均P>0.05)。与对照组相比,患者治疗前/后PON或HCY水平与血清维生素B12水平之间无统计学显著关系(P>0.05)。
本研究结果不支持抗氧化酶PON和ARES在维生素B12缺乏及相关高同型半胱氨酸血症中起潜在作用的假设。我们的研究结果表明,PON和ARES在维生素B12缺乏的全身效应中不起作用。