Zheng Shuzi, Wu Hao, Ye Fangpeng, Xia Xuanping, Xia Shenglong, Lin Xiuqing, Wu Xiaoli, Jiang Lijia, Ding Ran, Jiang Yi
Department of Gastroenterology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2017 Oct 10;34(5):731-736. doi: 10.3760/cma.j.issn.1003-9406.2017.05.025.
To assess the association of transcobalamine II (TCN2) gene polymorphisms and serum levels of homocysteine (Hcy), vitamin B and folate with ulcerative colitis (UC) among Chinese patients.
For 397 UC patients and 574 controls, two single nucleotide polymorphisms of the TCN2 gene (rs1801198, rs9606756) were tested with an improved multiple ligase detection reaction method. Serum Hcy, vitamin B and folate were measured with an enzymatic cycling assay and an chemiluminescence immunoassay, respectively.
The allelic and genotypic frequencies of rs1801198 and rs9606756 did not differ significantly between the two groups (all P> 0.05). Compared with those of the control group, the frequencies of G allele and CG+GG genotype of rs1801198 were greater in patients with moderate and severe UC (both P< 0.05). The same conclusion may also be drawn for the G allele and AG genotype of rs9606756 (both P< 0.05). Compared with the controls, average Hcy level was enhanced in UC patients (P< 0.01), whereas average vitamin B and folate levels were decreased in UC patients (both P< 0.01). In both groups, the average level of Hcy was lower in individuals carrying CC of (rs1801198) than in those with CG+GG (both P< 0.05). A similar conclusion was also drawn for individuals with AA of rs9606756 when compared with those carrying AG(both P< 0.05). Compared with patients with mild UC, average Hcy level was increased in those with moderate and severe UC (P< 0.01), while average vitamin B and folate levels were decreased in those with moderate and severe UC (both P< 0.01). The prevalence of hyperhomocysteinemia(HHcy), vitamin B deficiency and folate deficiency was greater in UC patients than in controls (all P< 0.01). In UC patients, the level of Hcy was negatively correlated with those of vitamin B (P< 0.01), albumin(P< 0.01), red blood cells(P< 0.01) and platelet (P< 0.05), but positively correlated with white blood cells(P< 0.01) and Mayo score (P< 0.01). Both HHcy and folate deficiency were independent risk factors for UC (OR=4.173, OR=5.206, both P< 0.01).
TCN2 (rs1801198, rs9606756) variations, as well as serum levels of Hcy, vitamin B and folate, are correlated with UC. Both HHcy and folate deficiency are independent risk factors for UC.
评估中国溃疡性结肠炎（UC）患者中钴胺素II（TCN2）基因多态性及血清同型半胱氨酸（Hcy）、维生素B和叶酸水平与UC的相关性。
采用改良多重连接检测反应法对397例UC患者和574例对照者进行TCN2基因的两个单核苷酸多态性（rs1801198、rs9606756）检测。分别采用酶循环法和化学发光免疫分析法测定血清Hcy、维生素B和叶酸水平。
两组间rs1801198和rs9606756的等位基因及基因型频率差异无统计学意义（均P>0.05）。与对照组相比，中度和重度UC患者中rs1801198的G等位基因及CG + GG基因型频率更高（均P<0.05）。rs9606756的G等位基因及AG基因型也得出相同结论（均P<0.05）。与对照组相比，UC患者的平均Hcy水平升高（P<0.01），而UC患者的平均维生素B和叶酸水平降低（均P<0.01）。两组中，rs1801198携带CC基因型个体的平均Hcy水平低于携带CG + GG基因型个体（均P<0.05）。rs9606756携带AA基因型个体与携带AG基因型个体相比也得出类似结论（均P<0.05）。与轻度UC患者相比，中度和重度UC患者的平均Hcy水平升高（P<0.01），而中度和重度UC患者的平均维生素B和叶酸水平降低（均P<0.01）。UC患者高同型半胱氨酸血症（HHcy）、维生素B缺乏和叶酸缺乏的患病率高于对照组（均P<0.01）。在UC患者中，Hcy水平与维生素B（P<0.01）、白蛋白（P<0.01）、红细胞（P<0.01）和血小板（P<0.05）水平呈负相关，但与白细胞（P<0.01）和梅奥评分（P<0.01）呈正相关。HHcy和叶酸缺乏均为UC的独立危险因素（OR = 4.173，OR = 5.206，均P<0.01）。
TCN2（rs1801198、rs9606756）变异以及血清Hcy、维生素B和叶酸水平与UC相关。HHcy和叶酸缺乏均为UC的独立危险因素。
