基于重组磷蛋白的单血清稀释酶联免疫吸附测定法用于新城疫病毒的快速血清学检测

Recombinant phosphoprotein based single serum dilution ELISA for rapid serological detection of Newcastle disease virus.

作者信息

Das Moushumee, Kumar Sachin

机构信息

Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.

出版信息

J Virol Methods. 2015 Dec 1;225:64-9. doi: 10.1016/j.jviromet.2015.09.003. Epub 2015 Sep 11.

DOI:10.1016/j.jviromet.2015.09.003

PMID:26367196

Abstract

Newcastle disease virus (NDV) is the causative agent of a highly contagious disease in avian species. All strains of NDV belong to avian paramyxovirus serotype-1. The disease is endemic in different parts of the world and vaccination is the only way to protect birds from NDV infection. The virus non-structural phosphoprotein (P) is the second most abundant protein and a major modulator of viral replication. Although P protein shows lesser evolutionary divergence among NDV isolates, it is known to be highly divergent among different avian paramyxovirus serotypes. In the present study, a recombinant P protein based single serum dilution ELISA was developed which showed better sensitivity, specificity and accuracy as compared to conventional methods for NDV detection. The recombinant P protein based ELISA could be an alternative to existing diagnostics against NDV infection in chickens.

摘要

新城疫病毒（NDV）是禽类一种高度传染性疾病的病原体。所有NDV毒株都属于禽副粘病毒1型。该病在世界不同地区呈地方性流行，接种疫苗是保护禽类免受NDV感染的唯一方法。病毒非结构磷蛋白（P）是含量第二丰富的蛋白，也是病毒复制的主要调节因子。尽管P蛋白在NDV分离株之间的进化差异较小，但已知在不同禽副粘病毒血清型之间差异很大。在本研究中，开发了一种基于重组P蛋白的单血清稀释ELISA，与传统的NDV检测方法相比，该方法具有更好的敏感性、特异性和准确性。基于重组P蛋白的ELISA可能是现有鸡NDV感染诊断方法的一种替代方法。