臭氧对感染根部的外生菌根真菌的火炬松苗的影响。

Effects of tropospheric ozone on loblolly pine seedlings inoculated with root infecting ophiostomatoid fungi.

机构信息

Forest Health Dynamics Laboratory, School of Forestry and Wildlife Sciences, Auburn University, Auburn, AL, USA.

School of Forestry and Wildlife Sciences, Auburn University, Auburn, AL, USA.

出版信息

Environ Pollut. 2015 Dec;207:130-7. doi: 10.1016/j.envpol.2015.08.053. Epub 2015 Sep 12.

DOI:10.1016/j.envpol.2015.08.053

PMID:26367706

Abstract

Seedlings from four loblolly pine (Pinus taeda L.) families were exposed in open-top chambers to charcoal-filtered air (CF), non-filtered air (NF) or air amended with ozone to 2 times ambient (2×). Two of the families used were selected for their tolerance to fungi associated with Southern Pine Decline while two were selected for their susceptibility. Seedlings were treated with five inoculation treatments: no wound (NW), wound only (W), wound + media (WM), Grosmannia huntii (GH) and Leptographium terebrantis (LT). After 118 days of exposure (AOT40 = 31 ppm-hr(-1) for 2× ozone) seedling volume, dry matter, chlorophyll content, water potential and lesions were measured and analyzed using ANOVA procedures. Our results indicate that seedlings selected for their susceptibility to root infecting ophiostomatoid fungi were also more sensitive to ozone. Overall lesion length was greater on seedlings exposed to elevated ozone concentrations but was not specific to either root infecting ophiostomatoid fungi.

摘要

从四个火炬松（Pinus taeda L.）家系中选择幼苗，在开顶式气室中暴露于经过活性炭过滤的空气（CF）、未经过滤的空气（NF）或臭氧浓度增加到环境水平 2 倍的空气中（2×）。选择了两个家系是因为它们对与南方松衰退相关的真菌具有耐受性，而选择了另外两个家系是因为它们具有易感性。幼苗接受了五种接种处理：无伤口（NW）、仅伤口（W）、伤口+培养基（WM）、 Grosmannia huntii（GH）和 Leptographium terebrantis（LT）。暴露 118 天后（AOT40 = 31 ppm-hr(-1) 持续 2×臭氧），测量并使用方差分析程序分析幼苗体积、干物质、叶绿素含量、水势和病变。我们的结果表明，对根侵染奥氏体真菌敏感的幼苗对臭氧也更敏感。暴露于高浓度臭氧的幼苗的总病变长度更大，但与根侵染奥氏体真菌无关。