Peragón Juan, Rufino-Palomares Eva E, Muñoz-Espada Irene, Reyes-Zurita Fernando J, Lupiáñez José A
Biochemistry and Molecular Biology Section, Department of Experimental Biology, University of Jaén, Campus Las Lagunillas, E-23071 Jaén, Spain.
Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Campus Fuentenueva, E-18001 Granada, Spain.
Int J Mol Sci. 2015 Sep 9;16(9):21681-94. doi: 10.3390/ijms160921681.
Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have important properties for health and disease prevention. MA selectively inhibits cell proliferation of the HT29 human colon-cancer cell line by inducing selective apoptosis. For measuring the MA and OA concentration inside the cell and in the culture medium, a new HPLC-MS procedure has been developed. With this method, a determination of the amount of MA and OA incorporated into HT29 and HepG2 human cancer-cell lines incubated with different concentrations of MA corresponding to 50% growth inhibitory concentration (IC50), IC50/2, IC50/4, and IC50/8 has been made. The results demonstrate that this method is appropriate for determining the MA and OA concentration in different types of cultured cells and reveals the specific dynamics of entry of MA into HT29 and HepG2 cells.
齐墩果酸（MA）和橄榄酸（OA）是橄榄油中主要的三萜酸，对健康和疾病预防具有重要特性。MA通过诱导选择性凋亡来选择性抑制HT29人结肠癌细胞系的细胞增殖。为了测量细胞内和培养基中MA和OA的浓度，已开发出一种新的高效液相色谱 - 质谱法（HPLC - MS）。使用该方法，测定了与50%生长抑制浓度（IC50）、IC50/2、IC50/4和IC50/8相对应的不同浓度MA孵育的HT29和HepG2人癌细胞系中MA和OA的掺入量。结果表明，该方法适用于测定不同类型培养细胞中MA和OA的浓度，并揭示了MA进入HT29和HepG2细胞的特定动力学。
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