Key Laboratory of Agro-Environment in the Tropics, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
Molecules. 2024 Sep 5;29(17):4216. doi: 10.3390/molecules29174216.
Many naturally occurring chemical metabolites with significant cytotoxic activities have been isolated from medicinal plants and have become the leading hotspot of anti-cancer research in recent years. Mart. et Gal is used as a folk medicine in South China to treat or assist in the treatment of liver disease, ulcers, and edema. But its chemical constituents have not been fully investigated yet. This study aimed to assess the cytotoxicity of , which was chemically characterized by chromatography-mass spectrometry methods. The results showed that the 95% ethanol extract of has marked inhibitory effects on five human cancer cell lines (HL-60, A549, SMMC-7721, MDA-MB-231, and SW480), with IC values ranging from 15.8 to 40.0 μg/mL. A total of 64 compounds were identified by ultra-high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) and gas chromatograph-mass spectroscopy (GC-MS) analysis of crude extract. Among them, kaempferol, quercetin, rosmarinic acid, squalene, and campesterol were found to be abundant and might be the major metabolites involved to its bioactivity. The cytotoxic characterization and metabolite profiling of displayed in this research provides scientific evidence to support its use as medicinal properties.
许多具有显著细胞毒性的天然存在的化学代谢物已从药用植物中分离出来，并成为近年来抗癌研究的主要热点。马缨丹在华南地区被用作民间药物，用于治疗或辅助治疗肝脏疾病、溃疡和水肿。但其化学成分尚未得到充分研究。本研究旨在评估马缨丹的细胞毒性，该化合物通过色谱-质谱联用方法进行化学表征。结果表明，马缨丹 95%乙醇提取物对五种人癌细胞系（HL-60、A549、SMMC-7721、MDA-MB-231 和 SW480）具有明显的抑制作用，IC 值范围为 15.8-40.0 μg/mL。通过超高效液相色谱-四极杆飞行时间质谱联用（UPLC-QTOF-MS）和气相色谱-质谱联用（GC-MS）分析粗提物，共鉴定出 64 种化合物。其中，山奈酚、槲皮素、迷迭香酸、角鲨烯和菜油甾醇含量丰富，可能是其生物活性的主要代谢物。本研究中对马缨丹的细胞毒性特征和代谢物分析为其药用价值提供了科学依据。
