Nakaya H, Kamoi K
Nihon Shishubyo Gakkai Kaishi. 1989 Jun;31(2):462-90. doi: 10.2329/perio.31.462.
This study describes the distribution of fibronectin and laminin in periodontal tissue of rats after a flap operation. After full thickness flaps were raised, the roots were surgically exposed and planed. Animals were sacrificed at 12 hours, and 1, 3, 5, 7, 14, 28 and 56 days after the wounding. The block specimens were fixed in formalin, decalcified with EDTA, made into serial paraffin sections, and examined after hematoxylin and eosin staining, after Masson-trichrome staining and by indirect immunofluorescence for the presence of fibronectin and laminin. After the wounding, fibronectin was detected in the fibrin clot, and the migratory epithelial cells crossed over this fibrin clot (12 hours-5 days). Fibronectin was deposited heavily in the granulation tissue. When the gingival connective tissue had matured, fibronectin diminished (5-14 days). On the root surface, a layer of fibronectin was present in the region where connective tissue fibers were oriented parallel to the root surface, while no fibronectin was seen at the site of reattachment of the regenerated collagen bundle (14-56 days). Laminin was present in the basement membrane of normal epithelium and blood vessels, but was absent from the internal basal lamina. After the wounding, laminin was absent from the basement membrane zone of the distal site of the migrating epithelium (1-3 days). Upon completion of wound reepithelialization at 5-7 days after wounding, laminin reappeared throughout the basement membrane except the internal basal lamina. These results suggest that fibronectin may be important in the regeneration of epithelium, connective tissue and connective tissue attachment during repair by functioning as an extracellular provisional matrix for migrating cells. On the other hand laminin may be important in maintaining the normal epithelium.
本研究描述了翻瓣手术后大鼠牙周组织中纤连蛋白和层粘连蛋白的分布情况。掀起全厚瓣后，手术暴露并平整牙根。在创伤后12小时、1天、3天、5天、7天、14天、28天和56天处死动物。将块状标本用福尔马林固定，用乙二胺四乙酸脱钙，制成连续石蜡切片，经苏木精-伊红染色、Masson三色染色及间接免疫荧光法检测纤连蛋白和层粘连蛋白的存在情况。创伤后，在纤维蛋白凝块中检测到纤连蛋白，迁移的上皮细胞穿过该纤维蛋白凝块（12小时至5天）。纤连蛋白大量沉积在肉芽组织中。当牙龈结缔组织成熟时，纤连蛋白减少（5至14天）。在根面上，结缔组织纤维与根面平行排列的区域存在一层纤连蛋白，而在再生胶原束重新附着的部位未见纤连蛋白（14至56天）。层粘连蛋白存在于正常上皮和血管的基底膜中，但内基膜中不存在。创伤后，迁移上皮远端部位的基底膜区无层粘连蛋白（1至3天）。创伤后5至7天伤口重新上皮化完成时，除内基膜外，层粘连蛋白重新出现在整个基底膜中。这些结果表明，纤连蛋白在修复过程中作为迁移细胞的细胞外临时基质，可能在上皮、结缔组织和结缔组织附着的再生中起重要作用。另一方面，层粘连蛋白可能在维持正常上皮方面起重要作用。
