Czerwiecki L, Giryn H
Rocz Panstw Zakl Hig. 1989;40(4-6):284-90.
A simple method is described of identification and determination of deoxynivalenol (DON), nivalenol (NIV), diacetoxyscirpenol (DAS) and T-2 toxin in cereals. Chloroform-ethanol extracts were purified in columns filled with active charcoal, aluminium oxide and celite and were analysed by the method of thin-layer chromatography. The results were evaluated exposing fluorescent trichothecene derivatives to ultraviolet light, after they had been obtained on a chromatographic plate with aluminium chloride and sulphuric acid. The detectability of the method for various toxins was: DON--37, NIV--100, DAS--50, and T-2 toxin--100 mcg/kg.
描述了一种鉴定和测定谷物中脱氧雪腐镰刀菌烯醇（DON）、雪腐镰刀菌烯醇（NIV）、二醋酸藨草镰刀菌烯醇（DAS）和T-2毒素的简单方法。氯仿-乙醇提取物在填充有活性炭、氧化铝和硅藻土的柱中进行纯化，并通过薄层色谱法进行分析。在使用氯化铝和硫酸的色谱板上获得荧光单端孢霉烯衍生物后，通过将其暴露于紫外光来评估结果。该方法对各种毒素的检测限为：DON——37、NIV——100、DAS——50和T-2毒素——100微克/千克。
