Frongia Giovanni, Nickkholgh Arash, Hafezi M Mohammad Reza, Arvin Jalal, Saffari Arash, Golriz Mohammad, Aydin Esvad, Weih Sandra, Kessler Markus, Emami Golnaz, Garoussi Camelia, Okun Jürgen G, Schmidt Kathrin, Thiel Christian, Brune Maik, Günther Patrick, Holland-Cunz Stefan, Mehrabi Arianeb
a Division of Pediatric Surgery, University Hospital of Heidelberg , Heidelberg , Germany.
b Department of General, Visceral and Transplantation Surgery, University Hospital of Heidelberg , Heidelberg , Germany.
J Invest Surg. 2016;29(1):57-65. doi: 10.3109/08941939.2015.1057304. Epub 2015 Sep 16.
Insufficient data are available to determine the most suitable extent of intestinal resection required to induce short-bowel syndrome (SBS) in pigs. This study aimed to compare the three main SBS-models published.
A 75%, 90%, or 100% mid-intestinal resection was performed in groups of n = 5 pigs each. Clinical (body weight, stool consistency) and biochemical (serum eletrolytes, citrulline, albumin, prealbumin, and transferrin) parameters were determined daily, functional (D-xylose resorption) and histological (intestinal villus length) parameters were determined after 2 weeks. A t-test and ANOVA were used for statistical analysis.
Only in the 100% group, we observed a persistent weight loss (13.6 ± 3.8%) and diarrhea, as well as a decrease in prealbumin-levels (41%) and transferrin levels (33%). Serum electrolytes remained stable in all groups during the observation period. Citrulline stabilized at different levels (100% group 13.9 ± 1.0 μmol/L; 90% group 18.8 ± 1.0 μmol/L; 75% group 26.3 ± 1.4 μmol/L; all p < .05). D-xylose resorption was lowest in the 100%, followed by 90% and 75% group (100% group 32.8 ± 4.9 mg/L; 90% group 50.0 ± 19.6 mg/L; 75% group 57.8 ± 8.8 mg/L; p = .393). Intestinal villus length decreased in all groups (100% group 11.0%; 90% group 14.0%; 75% group 19.1%).
75% intestinal resection is less suitable as an SBS model, as animals tend to recover remarkably. The 90% model is suitable for longer-term studies, as animals might survive longer due to partial compensation. Due to severe nutritional, biochemical, and physiological derangements, the 100% model can only be used for acute experiments and those immediately followed by small bowel transplantation.
目前尚无足够数据来确定诱导猪短肠综合征（SBS）所需的最合适肠道切除范围。本研究旨在比较已发表的三种主要SBS模型。
每组5头猪，分别进行75%、90%或100%的中肠切除术。每天测定临床参数（体重、粪便稠度）和生化参数（血清电解质、瓜氨酸、白蛋白、前白蛋白和转铁蛋白），2周后测定功能参数（D-木糖吸收）和组织学参数（肠绒毛长度）。采用t检验和方差分析进行统计分析。
仅在100%切除组中，观察到持续体重减轻（13.6±3.8%）和腹泻，以及前白蛋白水平降低（41%）和转铁蛋白水平降低（33%）。观察期内所有组血清电解质均保持稳定。瓜氨酸在不同水平稳定（100%切除组13.9±1.0 μmol/L；90%切除组18.8±1.0 μmol/L；75%切除组26.3±1.4 μmol/L；所有p<0.05）。D-木糖吸收在100%切除组最低，其次是90%切除组和75%切除组（100%切除组32.8±4.9 mg/L；90%切除组50.0±19.6 mg/L；75%切除组57.8±8.8 mg/L；p = 0.393）。所有组肠绒毛长度均缩短（100%切除组11.0%；90%切除组14.0%；75%切除组19.1%）。
75%肠道切除不太适合作为SBS模型，因为动物往往恢复显著。90%切除模型适用于长期研究，因为动物可能因部分代偿而存活更长时间。由于严重的营养、生化和生理紊乱，100%切除模型仅可用于急性实验以及紧接着进行小肠移植的实验。
