Wang Xin, Oh MyeongWon, Sakata Katsumi, Komatsu Setsuko
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan; National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
J Proteomics. 2016 Jan 1;130:42-55. doi: 10.1016/j.jprot.2015.09.007. Epub 2015 Sep 12.
Growth in the early stage of soybean is markedly inhibited under flooding and drought stresses. To explore the responsive mechanisms of soybean, temporal protein profiles of root tip under flooding and drought stresses were analyzed using gel-free/label-free proteomic technique. Root tip was analyzed because it was the most sensitive organ against flooding, and it was beneficial to root penetration under drought. UDP glucose: glycoprotein glucosyltransferase was decreased and increased in soybean root under flooding and drought, respectively. Temporal protein profiles indicated that fermentation and protein synthesis/degradation were essential in root tip under flooding and drought, respectively. In silico protein-protein interaction analysis revealed that the inductive and suppressive interactions between S-adenosylmethionine synthetase family protein and B-S glucosidase 44 under flooding and drought, respectively, which are related to carbohydrate metabolism. Furthermore, biotin/lipoyl attachment domain containing protein and Class II aminoacyl tRNA/biotin synthetases superfamily protein were repressed in the root tip during time-course stresses. These results suggest that biotin and biotinylation might be involved in energy management to cope with flooding and drought in early stage of soybean-root tip.
大豆生长早期在淹水和干旱胁迫下会受到显著抑制。为探究大豆的响应机制,采用无凝胶/无标记蛋白质组学技术分析了淹水和干旱胁迫下根尖的蛋白质动态谱。选择根尖进行分析是因为它是对淹水最敏感的器官,并且在干旱条件下有利于根系穿透。UDP葡萄糖:糖蛋白葡糖基转移酶在大豆根淹水和干旱条件下分别降低和升高。蛋白质动态谱表明,发酵以及蛋白质合成/降解分别在淹水和干旱条件下的根尖中至关重要。通过计算机模拟的蛋白质-蛋白质相互作用分析表明,S-腺苷甲硫氨酸合成酶家族蛋白与β-葡萄糖苷酶44在淹水和干旱条件下分别存在诱导和抑制相互作用,这与碳水化合物代谢有关。此外,在胁迫过程中,根尖中含生物素/硫辛酰附着结构域蛋白和II类氨酰tRNA/生物素合成酶超家族蛋白受到抑制。这些结果表明,生物素和生物素化可能参与了大豆根尖早期应对淹水和干旱的能量管理。