Chang Wei, Zhang Yan, Ping Yuan, Li Kun, Qi Dan-Dan, Song Fu-Qiang
Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, Harbin, China.
Jiaxiang Industrial Technology Research Institute of Heilongjiang University, Jinin, China.
Front Plant Sci. 2023 Jan 10;13:1098260. doi: 10.3389/fpls.2022.1098260. eCollection 2022.
Soil salinization has become one of the most serious environmental issues globally. Excessive accumulation of soluble salts will adversely affect the survival, growth, and reproduction of plants. Elaeagnus angustifolia L., commonly known as oleaster or Russian olive, has the characteristics of tolerance to drought and salt. Arbuscular mycorrhizal (AM) fungi are considered to be bio-ameliorator of saline soils that can enhance the salt tolerance of the host plants. However, there is little information on the root proteomics of AM plants under salt stress.
In this study, a label-free quantitative proteomics method was employed to identify the differentially abundant proteins in AM E. angustifolia seedlings under salt stress.
The results showed that a total of 170 proteins were significantly differentially regulated in E.angustifolia seedlings after AMF inoculation under salt stress. Mycorrhizal symbiosis helps the host plant E. angustifolia to respond positively to salt stress and enhances its salt tolerance by regulating the activities of some key proteins related to amino acid metabolism, lipid metabolism, and glutathione metabolism in root tissues.
Aspartate aminotransferase, dehydratase-enolase-phosphatase 1 (DEP1), phospholipases D, diacylglycerol kinase, glycerol-3-phosphate O-acyltransferases, and gamma-glutamyl transpeptidases may play important roles in mitigating the detrimental effect of salt stress on mycorrhizal E. angustifolia . In conclusion, these findings provide new insights into the salt-stress tolerance mechanisms of AM E. angustifolia seedlings and also clarify the role of AM fungi in the molecular regulation network of E. angustifolia under salt stress.
土壤盐渍化已成为全球最严重的环境问题之一。可溶性盐的过度积累会对植物的生存、生长和繁殖产生不利影响。沙枣，俗称沙棘或俄罗斯橄榄，具有耐旱和耐盐的特性。丛枝菌根（AM）真菌被认为是盐渍土壤的生物改良剂，能够提高宿主植物的耐盐性。然而，关于盐胁迫下AM植物根系蛋白质组学的信息却很少。
在本研究中，采用无标记定量蛋白质组学方法来鉴定盐胁迫下AM沙枣幼苗中差异丰富的蛋白质。
结果表明，盐胁迫下接种AMF后，沙枣幼苗中共有170种蛋白质受到显著差异调节。菌根共生有助于宿主植物沙枣对盐胁迫做出积极响应，并通过调节根组织中一些与氨基酸代谢、脂质代谢和谷胱甘肽代谢相关的关键蛋白质的活性来提高其耐盐性。
天冬氨酸转氨酶、脱水酶-烯醇酶-磷酸酶1（DEP1）、磷脂酶D、二酰基甘油激酶、甘油-3-磷酸O-酰基转移酶和γ-谷氨酰转肽酶可能在减轻盐胁迫对菌根化沙枣的有害影响方面发挥重要作用。总之，这些发现为AM沙枣幼苗的盐胁迫耐受机制提供了新的见解，也阐明了AM真菌在盐胁迫下沙枣分子调控网络中的作用。
