Zhu Xuewei, Sun Le, Wang Yusheng
a Department of Otolaryngology, Head & Neck Surgery , China-Japan Union Hospital, Jilin University , Changchun , PR China and.
b Department of Otolaryngology, Head & Neck Surgery , First Hospital of Jilin University , Changchun , PR China.
Acta Otolaryngol. 2016;136(1):87-94. doi: 10.3109/00016489.2015.1082192. Epub 2015 Sep 18.
The current study confirmed the significant high mobility group box 1 (HMGB1) was promoted in human nasopharyngeal carcinoma (NPC) tissues by Epstein-Barr virus (EBV) infection, in association with the malignant status of NPC, and promoted the proliferation NPC cells RAGE-dependently.
The present study was to examine the association of HMGB1 over-expression in human NPC with the EBV-positivity and to determine the regulatory role of HMGB1 on the proliferation of NPC cells in vitro.
Real-time PCR and Western blotting were utilized to examine the HMGB1 expression. EBV infection in CNE-2 cells was performed to investigate the HMGB1 promotion by EBV infection. RNA interference technology was utilized for the RAGE knockout.
It was demonstrated that HMGB1 was significantly higher in both mRNA and protein levels in the EBV-positive NPC tissues, in marked association with the malignant status of NPC, and with the LMP1 DNA level in EBV-positive NPC samples. In addition, the MTT assay, growth curve, and the colony forming assay confirmed the promotion by HMGB1 to the proliferation of CNE-2 cells, depending on RAGE.
当前研究证实，在人鼻咽癌（NPC）组织中，爱泼斯坦-巴尔病毒（EBV）感染可促进高迁移率族蛋白B1（HMGB1）的表达，这与NPC的恶性状态相关，且HMGB1通过RAGE依赖性方式促进NPC细胞增殖。
本研究旨在探讨人NPC中HMGB1过表达与EBV阳性的相关性，并确定HMGB1在体外对NPC细胞增殖的调控作用。
采用实时PCR和蛋白质印迹法检测HMGB1的表达。通过对CNE-2细胞进行EBV感染，研究EBV感染对HMGB1的促进作用。利用RNA干扰技术敲除RAGE。
结果表明，EBV阳性NPC组织中HMGB1的mRNA和蛋白水平均显著升高，这与NPC的恶性状态以及EBV阳性NPC样本中的LMP1 DNA水平显著相关。此外，MTT法、生长曲线和集落形成试验证实，HMGB1可通过RAGE促进CNE-2细胞增殖。
